Document Detail


Cell membrane damage induced by phenolic acids on wine lactic acid bacteria.
MedLine Citation:
PMID:  19733929     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this work was to investigate the effect of phenolic acids on cell membrane permeability of lactic acid bacteria from wine. Several phenolic acids were tested for their effects on the cell membrane of Oenococcus oeni and Lactobacillus hilgardii by measuring potassium and phosphate efflux, proton influx and by assessing culture viability employing a fluorescence technique based on membrane integrity. The experimental results indicate that hydroxycinnamic acids (p-coumaric, caffeic and ferulic acids) induce greater ion leakages and higher proton influx than hydroxybenzoic acids (p-hydroxibenzoic, protocatechuic, gallic, vanillic, and syringic acids). Among the hydroxycinnamic acids, p-coumaric acid showed the strongest effect. Moreover, the exposure of cells to phenolic acids caused a significant decrease in cell culture viability, as measured by the fluorescence assay, in both tested strains. The results agree with previous results obtained in growth experiments with the same strains. Generally, phenolic acids increased the cell membrane permeability in lactic acid bacteria from wine. The different effects of phenolic acids on membrane permeability could be related to differences in their structure and lipophilic character.
Authors:
F M Campos; J A Couto; A R Figueiredo; I V T??th; A O S S Rangel; T A Hogg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-04
Journal Detail:
Title:  International journal of food microbiology     Volume:  135     ISSN:  1879-3460     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-09-28     Completed Date:  2010-01-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  144-51     Citation Subset:  IM    
Affiliation:
CBQF/Escola Superior de Biotecnologia, Universidade Cat??lica Portuguesa, Rua Dr. Ant??nio Bernardino de Almeida, 4200-072 Porto, Portugal. fmcampos@mail.esb.ucp.pt
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MeSH Terms
Descriptor/Qualifier:
Bacterial Structures / drug effects*
Biological Transport
Cell Membrane / drug effects*
Coumaric Acids / pharmacology
Fluorescence
Hydrogen-Ion Concentration
Hydroxybenzoic Acids / pharmacology
Lactobacillus / drug effects*,  metabolism
Microbial Viability
Oenococcus / drug effects*,  metabolism
Permeability
Phenols / pharmacology*
Potassium / metabolism
Wine / microbiology*
Chemical
Reg. No./Substance:
0/Coumaric Acids; 0/Hydroxybenzoic Acids; 0/Phenols; 7440-09-7/Potassium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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