Document Detail

Cell-mediated immunity to herpes simplex virus: specificity of cytotoxic T cells.
MedLine Citation:
PMID:  6969228     Owner:  NLM     Status:  MEDLINE    
This communication deals with the question of which of the viral antigens constitutes the targets for cytotoxic T lymphocytes (CTL) generated against herpes simplex virus type 1 (HSV-1). The approach used was, first, to compare cytotoxicity of CTL against target cells infected with virus in the presence of tunicamycin and 2-deoxy-D-glucose, which are known to inhibit glycoprotein synthesis, and second, to compare cytotoxicity of CTL against target cells infected with wild-type HSV-1 with that against target cells infected with a temperature-sensitive mutant of HSV-1 which, at the nonpermissive temperature, exhibits diminished glycoprotein synthesis. The results show that glycoprotein expression is required for the demonstration of cytotoxic activity of CTL. The level of cytotoxicity against the temperature-sensitive HSV-1 target at the nonpermissive temperature was reduced and correlated with the level of expression of the major envelope glycoprotein region (VP123; molecular weight = 123,000) at the target cell surface as measured serologically by antibody binding studies. The results were interpreted to indicate that HSV-1-induced glycoproteins are the target antigens for anti-HSV CTL and that the principal viral antigens recognized by the CTL may be glycoproteins of the VP123 region.
M J Lawman; R J Courtney; R Eberle; P A Schaffer; M K O'Hara; B T Rouse
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  30     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1980 Nov 
Date Detail:
Created Date:  1981-02-19     Completed Date:  1981-02-19     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  451-61     Citation Subset:  IM    
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MeSH Terms
Antigens, Viral / analysis
Cytotoxicity, Immunologic*
Deoxyglucose / pharmacology
Glycoproteins / immunology
H-2 Antigens
Herpes Simplex / immunology*
Immunity, Cellular*
L Cells (Cell Line)
T-Lymphocytes / immunology*
Tunicamycin / pharmacology
Viral Proteins / immunology
Grant Support
A1 14981//PHS HHS; CA 24564/CA/NCI NIH HHS
Reg. No./Substance:
0/Antigens, Viral; 0/Glycoproteins; 0/H-2 Antigens; 0/Viral Proteins; 11089-65-9/Tunicamycin; 154-17-6/Deoxyglucose

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