| Cell-mediated LDL oxidation: the impact of transition metals and transferrin. | |
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MedLine Citation:
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PMID: 16288727 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Activated monocytes release oxygen radicals by respiratory burst and oxidative damage can be accelerated by transition metals. We investigated the cell-mediated and metal-catalysed in vitro oxidation of low-density lipoproteins (LDL), as well as the impact of the metal-binding protein transferrin (Tf). LDL oxidation was measured by monitoring the increase in fluorescence (350/440 nm excitation/emission). Maximal respiratory burst by U937 cells was achieved after 96 h differentiation with retinoic acid and dihydroxyvitamin D3 followed by stimulation with opsonised zymosan. Addition of activated cells resulted in the LDL oxidation, even in the absence of transition metals. Moreover, activated cells greatly enhanced metal-catalysed oxidative modifications, especially in the presence of copper. By binding metals, Tf was able to strongly impair this process. In conclusion, by generating oxygen radicals, activated U937 cells were able to oxidise LDL. The oxidising process was most pronounced in the presence of copper and could be blocked by Tf. |
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Authors:
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Ann Van Campenhout; Elke Heytens; Christel Van Campenhout; Albert R Lagrou; Begoña Manuel-y-Keenoy |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-11-02 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 338 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2005 Dec |
Date Detail:
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Created Date: 2005-11-18 Completed Date: 2006-01-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 1617-24 Citation Subset: IM |
Affiliation:
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Laboratory of Endocrinology, University of Antwerp, Belgium. ann.vancampenhout@ua.ac.be |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Catalysis Cell Differentiation Cell Line, Tumor Copper / pharmacology* Humans Iron / pharmacology* Lipoproteins, LDL / metabolism* Oxidation-Reduction / drug effects Transferrin / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Lipoproteins, LDL; 11096-37-0/Transferrin; 7439-89-6/Iron; 7440-50-8/Copper |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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