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Cell-laden microengineered pullulan methacrylate hydrogels promote cell proliferation and 3D cluster formation.
MedLine Citation:
PMID:  21415929     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The ability to encapsulate cells in three-dimensional (3D) environments is potentially of benefit for tissue engineering and regenerative medicine. In this paper, we introduce pullulan methacrylate (PulMA) as a promising hydrogel platform for creating cell-laden microscale tissues. The hydration and mechanical properties of PulMA were demonstrated to be tunable through modulation of the degree of methacrylation and gel concentration. Cells encapsulated in PulMA exhibited excellent viability. Interestingly, while cells did not elongate in PulMA hydrogels, cells proliferated and organized into clusters, the size of which could be controlled by the hydrogel composition. By mixing with gelatin methacrylate (GelMA), the biological properties of PulMA could be enhanced as demonstrated by cells readily attaching to, proliferating, and elongating within the PulMA/GelMA composite hydrogels. These data suggest that PulMA hydrogels could be useful for creating complex, cell-responsive microtissues, especially for applications that require controlled cell clustering and proliferation.
Authors:
Hojae Bae; Amir F Ahari; Hyeongho Shin; Jason W Nichol; Che B Hutson; Mahdokht Masaeli; Su-Hwan Kim; Hug Aubin; Seda Yamanlar; Ali Khademhosseini
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Soft matter     Volume:  7     ISSN:  1744-6848     ISO Abbreviation:  -     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-3-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101295070     Medline TA:  Soft Matter     Country:  -    
Other Details:
Languages:  ENG     Pagination:  1903-1911     Citation Subset:  -    
Affiliation:
Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 65 Landsdowne Street, Rm 252, Cambridge, MA, 02139, USA.
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Grant Support
ID/Acronym/Agency:
R01 HL092836-02S1//NHLBI NIH HHS; R01 HL092836-03//NHLBI NIH HHS; R01 HL092836-04//NHLBI NIH HHS; RL1 DE019024-04//NIDCR NIH HHS; RL1 DE019024-05//NIDCR NIH HHS

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