Document Detail


Cell-laden microengineered gelatin methacrylate hydrogels.
MedLine Citation:
PMID:  20417964     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cellular microenvironment plays an integral role in improving the function of microengineered tissues. Control of the microarchitecture in engineered tissues can be achieved through photopatterning of cell-laden hydrogels. However, despite high pattern fidelity of photopolymerizable hydrogels, many such materials are not cell-responsive and have limited biodegradability. Here, we demonstrate gelatin methacrylate (GelMA) as an inexpensive, cell-responsive hydrogel platform for creating cell-laden microtissues and microfluidic devices. Cells readily bound to, proliferated, elongated, and migrated both when seeded on micropatterned GelMA substrates as well as when encapsulated in microfabricated GelMA hydrogels. The hydration and mechanical properties of GelMA were demonstrated to be tunable for various applications through modification of the methacrylation degree and gel concentration. The pattern fidelity and resolution of GelMA were high and it could be patterned to create perfusable microfluidic channels. Furthermore, GelMA micropatterns could be used to create cellular micropatterns for in vitro cell studies or 3D microtissue fabrication. These data suggest that GelMA hydrogels could be useful for creating complex, cell-responsive microtissues, such as endothelialized microvasculature, or for other applications that require cell-responsive microengineered hydrogels.
Authors:
Jason W Nichol; Sandeep T Koshy; Hojae Bae; Chang M Hwang; Seda Yamanlar; Ali Khademhosseini
Related Documents :
9434634 - Deregulation of collagen phagocytosis in aging human fibroblasts: effects of integrin e...
17502044 - New developments in fibroblast and myofibroblast biology: implications for fibrosis and...
16144464 - Poly(beta-hydroxybutyrate-co-beta-hydroxyvalerate) supports in vitro osteogenesis.
19768794 - Effects of cell concentration and collagen concentration on contraction kinetics and me...
23555634 - Inhibition of p53-dependent, but not p53-independent, cell death by u19 protein from hu...
2172694 - Apo forms of cytochrome c550 and cytochrome cd1 are translocated to the periplasm of pa...
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-04-24
Journal Detail:
Title:  Biomaterials     Volume:  31     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-05-24     Completed Date:  2010-08-23     Revised Date:  2011-08-01    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  5536-44     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Elsevier Ltd. All rights reserved.
Affiliation:
Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 65 Landsdowne Street, Cambridge, MA 02139, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Biocompatible Materials / chemistry
Cell Adhesion
Cell Movement
Cell Proliferation
Cells, Cultured
Gelatin / chemistry*
Humans
Hydrogels / chemistry*
Materials Testing
Methacrylates / chemistry*
Microfluidics / instrumentation
Molecular Structure
Surface Properties
Tissue Engineering
Tissue Scaffolds
Grant Support
ID/Acronym/Agency:
DE019024/DE/NIDCR NIH HHS; HL092836/HL/NHLBI NIH HHS; R01 EB008392-01A2/EB/NIBIB NIH HHS; R01 EB008392-02/EB/NIBIB NIH HHS; R01 HL092836-02/HL/NHLBI NIH HHS; R01 HL092836-02S1/HL/NHLBI NIH HHS; R01 HL092836-03/HL/NHLBI NIH HHS; R01 HL092836-04/HL/NHLBI NIH HHS; R21 EB009196-02/EB/NIBIB NIH HHS; RL1 DE019024-01/DE/NIDCR NIH HHS; RL1 DE019024-02/DE/NIDCR NIH HHS; RL1 DE019024-03/DE/NIDCR NIH HHS; RL1 DE019024-03S1/DE/NIDCR NIH HHS; RL1 DE019024-04/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 0/Hydrogels; 0/Methacrylates; 9000-70-8/Gelatin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The use of calcium phosphate nanoparticles encapsulating Toll-like receptor ligands and the antigen ...
Next Document:  Clinical observations of biofouling on PEO coated silicone hydrogel contact lenses.