Document Detail

Cell-laden microengineered gelatin methacrylate hydrogels.
MedLine Citation:
PMID:  20417964     Owner:  NLM     Status:  MEDLINE    
The cellular microenvironment plays an integral role in improving the function of microengineered tissues. Control of the microarchitecture in engineered tissues can be achieved through photopatterning of cell-laden hydrogels. However, despite high pattern fidelity of photopolymerizable hydrogels, many such materials are not cell-responsive and have limited biodegradability. Here, we demonstrate gelatin methacrylate (GelMA) as an inexpensive, cell-responsive hydrogel platform for creating cell-laden microtissues and microfluidic devices. Cells readily bound to, proliferated, elongated, and migrated both when seeded on micropatterned GelMA substrates as well as when encapsulated in microfabricated GelMA hydrogels. The hydration and mechanical properties of GelMA were demonstrated to be tunable for various applications through modification of the methacrylation degree and gel concentration. The pattern fidelity and resolution of GelMA were high and it could be patterned to create perfusable microfluidic channels. Furthermore, GelMA micropatterns could be used to create cellular micropatterns for in vitro cell studies or 3D microtissue fabrication. These data suggest that GelMA hydrogels could be useful for creating complex, cell-responsive microtissues, such as endothelialized microvasculature, or for other applications that require cell-responsive microengineered hydrogels.
Jason W Nichol; Sandeep T Koshy; Hojae Bae; Chang M Hwang; Seda Yamanlar; Ali Khademhosseini
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-04-24
Journal Detail:
Title:  Biomaterials     Volume:  31     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-05-24     Completed Date:  2010-08-23     Revised Date:  2014-09-22    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  5536-44     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Elsevier Ltd. All rights reserved.
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MeSH Terms
Biocompatible Materials / chemistry
Cell Adhesion
Cell Movement
Cell Proliferation
Cells, Cultured
Gelatin / chemistry*
Hydrogels / chemistry*
Materials Testing
Methacrylates / chemistry*
Microfluidics / instrumentation
Molecular Structure
Surface Properties
Tissue Engineering
Tissue Scaffolds
Grant Support
DE019024/DE/NIDCR NIH HHS; HL092836/HL/NHLBI NIH HHS; R01 EB008392/EB/NIBIB NIH HHS; R01 EB008392-01A2/EB/NIBIB NIH HHS; R01 EB008392-02/EB/NIBIB NIH HHS; R01 HL092836/HL/NHLBI NIH HHS; R01 HL092836-02/HL/NHLBI NIH HHS; R01 HL092836-02S1/HL/NHLBI NIH HHS; R01 HL092836-03/HL/NHLBI NIH HHS; R01 HL092836-04/HL/NHLBI NIH HHS; R21 EB009196/EB/NIBIB NIH HHS; R21 EB009196-02/EB/NIBIB NIH HHS; RL1 DE019024/DE/NIDCR NIH HHS; RL1 DE019024-01/DE/NIDCR NIH HHS; RL1 DE019024-02/DE/NIDCR NIH HHS; RL1 DE019024-03/DE/NIDCR NIH HHS; RL1 DE019024-03S1/DE/NIDCR NIH HHS; RL1 DE019024-04/DE/NIDCR NIH HHS
Reg. No./Substance:
0/Biocompatible Materials; 0/Hydrogels; 0/Methacrylates; 9000-70-8/Gelatin

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