| Cell labeling with the positive MR contrast agent Gadofluorine M. | |
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MedLine Citation:
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PMID: 17206428 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The purpose of this study was to label human monocytes with Gadofluorine M by simple incubation for subsequent cell depiction at 1.5 and 3 T. Gadofluorine M displays a high r(1) relaxivity and is spontaneously phagocytosed by macrophages. Human monocytes were incubated with Gadofluorine M-Cy at varying concentrations and incubation times and underwent MR imaging at 1.5 and 3 T at increasing time intervals after the labeling procedure. R1-relaxation rates and r1 relaxivities of the labeled cells and non-labeled controls were determined. Cellular contrast agent uptake was examined by fluorescence microscopy and quantified by ICP-AES. Efficient cell labeling was achieved after incubation of the cells with 25 mM Gd Gadofluorine M for 12 h, resulting in a maximal uptake of 0.3 fmol Gd/cell without impairment of cell viability. Fluorescence microscopy confirmed internalization of the fluorescent contrast agent by monocytes. The r1 relaxivity of the labeled cells was 137 mM(-1)s(-1) at 1.5 T and 80.46 mM(-1)s(-1) at 3 T. Imaging studies showed stable labeling for at least 7 days. Human monocytes can be effectively labeled for MR imaging with Gadofluorine M. Potential in vivo cell-tracking applications include targeting of inflammatory processes with Gadofluorine-labeled leukocytes or monitoring of stem cell therapies for the treatment of arthritis. |
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Authors:
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Tobias D Henning; Olaf Saborowski; Daniel Golovko; Sophie Boddington; Jan S Bauer; Yanjun Fu; Reinhard Meier; Hubertus Pietsch; Barbara Sennino; Donald M McDonald; Heike E Daldrup-Link |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2007-01-06 |
Journal Detail:
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Title: European radiology Volume: 17 ISSN: 0938-7994 ISO Abbreviation: Eur Radiol Publication Date: 2007 May |
Date Detail:
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Created Date: 2007-04-12 Completed Date: 2008-09-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9114774 Medline TA: Eur Radiol Country: Germany |
Other Details:
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Languages: eng Pagination: 1226-34 Citation Subset: IM |
Affiliation:
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Department of Radiology, University of California in San Francisco, 505 Parnassus Ave, San Francisco, CA, 94143, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cells, Cultured Contrast Media / pharmacokinetics* Humans Magnetic Resonance Imaging / methods* Microscopy, Confocal Monocytes / metabolism* Organometallic Compounds / pharmacokinetics* Spectrophotometry, Atomic Staining and Labeling |
| Grant Support | |
ID/Acronym/Agency:
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CA082923/CA/NCI NIH HHS; HL 59157/HL/NHLBI NIH HHS; HL-24136/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Contrast Media; 0/Organometallic Compounds; 0/gadofluorine M |
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