Document Detail

Cell kinetic characteristics in different parts of multicellular spheroids of human origin.
MedLine Citation:
PMID:  7197587     Owner:  NLM     Status:  MEDLINE    
The growth fraction, the cell cycle time, and the duration of the individual cell cycle phases were determined as a function of distance from the surface of multicellular spheroids of the human cell line NHIK 3025. The techniques employed were percentage of labelled mitoses and labelling index measurements after autoradiography and flow cytometric measurements of DNA histograms. To separate cell populations from the different parts of the spheroid, fractionated trypsinization was employed. The results were compared with corresponding values in NHIK 3025 cell populations grown as monolayer cultures. While practically all cells in exponentially growing monolayer populations are proliferating, the growth fraction was between 0.6 and 0.7 in the outer parts of the spheroid. The inner region was mainly occupied by a necrotic mass. The proliferating fraction of the recognizable cells in the inner region was slightly below 0.5. The mean cell cycle time of NHIK 3025 cells in monolayer culture is 18 hr. The mean cell cycle time of proliferating cells in the periphery of the spheroid was 30 hr, compared to 41 hr in the inner region (150 micrometer from the spheroid surface). All phases of the cell cycle were prolonged compared to populations of exponentially growing monolayer cells. Within each part of the spheroid the distribution of cell cycle times was considerably broadened compared with monolayer populations.
E Wibe; T Lindmo; O Kaalhus
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell and tissue kinetics     Volume:  14     ISSN:  0008-8730     ISO Abbreviation:  Cell Tissue Kinet     Publication Date:  1981 Nov 
Date Detail:
Created Date:  1982-01-20     Completed Date:  1982-01-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0174107     Medline TA:  Cell Tissue Kinet     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  639-51     Citation Subset:  IM    
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MeSH Terms
Cell Line
DNA, Neoplasm / analysis
Uterine Cervical Neoplasms / pathology*
Reg. No./Substance:
0/DNA, Neoplasm

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