| Cell interactions in the immune response in vitro. 3. Specific collaboration across a cell impermeable membrane. | |
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MedLine Citation:
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PMID: 5064225 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Tissue cultures with two compartments, separated by a cell impermeable nuclepore membrane (1 micro pore size), were used to investigate the mechanism of T-B lymphocyte cooperation. It was found that collaboration was as effective when the T and B lymphocyte populations were separated by the membrane as when they were mixed together. Critical tests were performed to verify that the membranes used were in fact cell impermeable. The specificity of the augmentation of the B cell response by various T cell populations was investigated. Only the response of B cells reactive to determinants on the same molecule as recognized by the T cells was augmented markedly. Specific activation of thymocytes by antigen was necessary for efficient collaboration across the membrane. The response of both unprimed and hapten-primed spleen cells was augmented by the T cell "factor" although, as expected, hapten-primed cells yielded greater responses. The T cell factor acted as efficiently if T cells were present or absent in the lower chamber. Thus the site of action of the T cell factor was not on other T cells, but was either on macrophages or the B cells themselves. The T cell-specific immunizing factor did not pass through dialysis membranes. The experiments reported here help rule out some of the possible theories of T-B cell collaboration. Clearly T-B cell contact was not necessary for successful cooperation to occur in this system. Possible theoretical interpretations of the results and their bearing on the detailed mechanism of T-B lymphocyte cooperation are discussed. |
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Authors:
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M Feldmann; A Basten |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: The Journal of experimental medicine Volume: 136 ISSN: 0022-1007 ISO Abbreviation: J. Exp. Med. Publication Date: 1972 Jul |
Date Detail:
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Created Date: 1972-08-10 Completed Date: 1972-08-10 Revised Date: 2010-06-22 |
Medline Journal Info:
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Nlm Unique ID: 2985109R Medline TA: J Exp Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 49-67 Citation Subset: AIM; IM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibody-Producing Cells* Antigens, Bacterial Antimycin A / pharmacology Cells, Cultured Dactinomycin / pharmacology Dialysis Dinitrophenols Erythrocytes / immunology Hemocyanin Hybridization, Genetic Immunity, Cellular* Isoantibodies Lymphocytes / immunology* Membranes, Artificial* Mice Mice, Inbred Strains Salmonella / immunology Sheep Spleen / cytology, immunology* Thymus Gland / cytology, immunology* |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Bacterial; 0/Dinitrophenols; 0/Isoantibodies; 0/Membranes, Artificial; 50-76-0/Dactinomycin; 642-15-9/Antimycin A; 9013-72-3/Hemocyanin |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Exp Med ISSN: 0022-1007 ISSN: 1540-9538 Publisher: The Rockefeller University Press |
Article Information Download PDF  Copyright © 1972 by The Rockefeller University Press Received Day: 12 Month: 3 Year: 1972 Print publication date: Day: 1 Month: 7 Year: 1972 Volume: 136 Issue: 1 First Page: 49 Last Page: 67 ID: 2139192 PubMed Id: 5064225 |
| CELL INTERACTIONS IN THE IMMUNE RESPONSE IN VITRO : III. SPECIFIC COLLABORATION ACROSS A CELL IMPERMEABLE MEMBRANE | |
| Marc Feldmann | |
| Antony Basten | |
| From The Walter and Eliza Hall Institute of Medical Research, Victoria 3050, Australia |
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