|Cell interactions in the immune response in vitro. 3. Specific collaboration across a cell impermeable membrane.|
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|PMID: 5064225 Owner: NLM Status: MEDLINE|
|Tissue cultures with two compartments, separated by a cell impermeable nuclepore membrane (1 micro pore size), were used to investigate the mechanism of T-B lymphocyte cooperation. It was found that collaboration was as effective when the T and B lymphocyte populations were separated by the membrane as when they were mixed together. Critical tests were performed to verify that the membranes used were in fact cell impermeable. The specificity of the augmentation of the B cell response by various T cell populations was investigated. Only the response of B cells reactive to determinants on the same molecule as recognized by the T cells was augmented markedly. Specific activation of thymocytes by antigen was necessary for efficient collaboration across the membrane. The response of both unprimed and hapten-primed spleen cells was augmented by the T cell "factor" although, as expected, hapten-primed cells yielded greater responses. The T cell factor acted as efficiently if T cells were present or absent in the lower chamber. Thus the site of action of the T cell factor was not on other T cells, but was either on macrophages or the B cells themselves. The T cell-specific immunizing factor did not pass through dialysis membranes. The experiments reported here help rule out some of the possible theories of T-B cell collaboration. Clearly T-B cell contact was not necessary for successful cooperation to occur in this system. Possible theoretical interpretations of the results and their bearing on the detailed mechanism of T-B lymphocyte cooperation are discussed.|
|M Feldmann; A Basten|
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|Type: In Vitro; Journal Article|
|Title: The Journal of experimental medicine Volume: 136 ISSN: 0022-1007 ISO Abbreviation: J. Exp. Med. Publication Date: 1972 Jul|
|Created Date: 1972-08-10 Completed Date: 1972-08-10 Revised Date: 2010-06-22|
Medline Journal Info:
|Nlm Unique ID: 2985109R Medline TA: J Exp Med Country: UNITED STATES|
|Languages: eng Pagination: 49-67 Citation Subset: AIM; IM|
|APA/MLA Format Download EndNote Download BibTex|
Antimycin A / pharmacology
Dactinomycin / pharmacology
Erythrocytes / immunology
Lymphocytes / immunology*
Mice, Inbred Strains
Salmonella / immunology
Spleen / cytology, immunology*
Thymus Gland / cytology, immunology*
|0/Antigens, Bacterial; 0/Dinitrophenols; 0/Isoantibodies; 0/Membranes, Artificial; 50-76-0/Dactinomycin; 642-15-9/Antimycin A; 9013-72-3/Hemocyanin|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Journal ID (nlm-ta): J Exp Med
Publisher: The Rockefeller University Press
Copyright © 1972 by The Rockefeller University Press
Received Day: 12 Month: 3 Year: 1972
Print publication date: Day: 1 Month: 7 Year: 1972
Volume: 136 Issue: 1
First Page: 49 Last Page: 67
PubMed Id: 5064225
|CELL INTERACTIONS IN THE IMMUNE RESPONSE IN VITRO : III. SPECIFIC COLLABORATION ACROSS A CELL IMPERMEABLE MEMBRANE|
|From The Walter and Eliza Hall Institute of Medical Research, Victoria 3050, Australia
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