Document Detail

Cell immobilization and xylitol production using sugarcane bagasse as raw material.
MedLine Citation:
PMID:  18025553     Owner:  NLM     Status:  MEDLINE    
Sugarcane bagasse pretreated by three different procedures (with 2% [v/v] polyethyleneimine (PEI), with 2% [w/v] NaOH, or with a sequence of NaOH and PEI) was used as cell immobilization carrier for xylitol production by Candida guilliermondii yeast. Fermentations using these pretreated carriers were performed in semidefined medium and in a hydrolysate medium produced from sugarcane bagasse hemicellulose. Sugarcane bagasse pretreated with NaOH was the best carrier obtained with respect to immobilization efficiency, because it was able to immobilize a major quantity of cells (0.30 g of cells/g of bagasse). Fermentation in semidefined medium using the NaOH-pretreated carrier attained a high efficiency of xylose-to-xylitol bioconversion (96% of the theoretical value). From hydrolysate medium, the bioconversion efficiency was lower (63%), probably owing to the presence of other substances in the medium that caused an inadequate mass transfer to the cells. In this fermentation medium, better results with relation to xylitol production were obtained by using PEI-pretreated carrier (xylose-to-xylitol bioconversion of 81% of the theoretical and volumetric productivity of 0.43 g/[L.h]). The results showed that sugarcane bagasse is a low-cost material with great potential for use as cell immobilization carrier in the fermentative process for xylitol production.
Silvio S Silva; Solange I Mussatto; Júlio C Santos; Diego T Santos; Juliana Polizel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  141     ISSN:  0273-2289     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:    2007 May-Jun
Date Detail:
Created Date:  2007-11-20     Completed Date:  2008-01-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  215-27     Citation Subset:  IM    
Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paolo, Estrada Municipal do Campinho Sin, Lorena, SP, CEP 12602-810, Brazil.
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MeSH Terms
Biotechnology / methods
Candida / metabolism*
Cells, Immobilized
Cellulose / chemistry,  metabolism*
Culture Media / metabolism
Microscopy, Electron, Scanning
Polysaccharides / chemistry
Saccharum / metabolism*
Xylitol / chemistry*
Reg. No./Substance:
0/Culture Media; 0/Polysaccharides; 8024-50-8/hemicellulose; 87-99-0/Xylitol; 9004-34-6/Cellulose; 9006-97-7/bagasse

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