Document Detail


Cell immobilization with polyurethane foam for retaining Trichoderma reesei cells during foam fractionation for cellulase collection.
MedLine Citation:
PMID:  19127442     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In situ affinity foam fractionation is a potential powerful tool for continuous, selective removal of products from bioprocesses. When evaluating its applicability to cellulase production by Trichoderma reesei fermentation, we encountered the difficulty of significant removal of fungal mycelia along with the cellulase. To solve this problem, cell immobilization using cut pieces of hydrophilic polyurethane (PU) foam was evaluated. Five commercial PU foams with different pore sizes and porosities were tested. Two were found to support good cell growth, cellulase production, and cell loading (about 0.6 g dry cells per g PU). The PU-immobilized mycelia were successfully retained in the foaming process.
Authors:
Qin Zhang; Chi-Ming Lo; Lu-Kwang Ju
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-01-06
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  156     ISSN:  1559-0291     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-09     Completed Date:  2009-05-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  12-23     Citation Subset:  IM    
Affiliation:
Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, OH 44325-3906, USA.
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MeSH Terms
Descriptor/Qualifier:
Cells, Immobilized / metabolism
Cellulase / biosynthesis*,  metabolism
Feasibility Studies
Fermentation
Polyurethanes / metabolism*
Trichoderma / growth & development,  metabolism*
Chemical
Reg. No./Substance:
0/Polyurethanes; 9009-54-5/polyurethane foam; EC 3.2.1.4/Cellulase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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