| Cell immobilization with polyurethane foam for retaining Trichoderma reesei cells during foam fractionation for cellulase collection. | |
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MedLine Citation:
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PMID: 19127442 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In situ affinity foam fractionation is a potential powerful tool for continuous, selective removal of products from bioprocesses. When evaluating its applicability to cellulase production by Trichoderma reesei fermentation, we encountered the difficulty of significant removal of fungal mycelia along with the cellulase. To solve this problem, cell immobilization using cut pieces of hydrophilic polyurethane (PU) foam was evaluated. Five commercial PU foams with different pore sizes and porosities were tested. Two were found to support good cell growth, cellulase production, and cell loading (about 0.6 g dry cells per g PU). The PU-immobilized mycelia were successfully retained in the foaming process. |
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Authors:
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Qin Zhang; Chi-Ming Lo; Lu-Kwang Ju |
Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, U.S. Gov't, Non-P.H.S. Date: 2009-01-06 |
Journal Detail:
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Title: Applied biochemistry and biotechnology Volume: 156 ISSN: 1559-0291 ISO Abbreviation: Appl. Biochem. Biotechnol. Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-04-09 Completed Date: 2009-05-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8208561 Medline TA: Appl Biochem Biotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 12-23 Citation Subset: IM |
Affiliation:
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Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, OH 44325-3906, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cells, Immobilized
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metabolism Cellulase / biosynthesis*, metabolism Feasibility Studies Fermentation Polyurethanes / metabolism* Trichoderma / growth & development, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Polyurethanes; 9009-54-5/polyurethane foam; EC 3.2.1.4/Cellulase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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