| Cell growth inhibition, G2/M cell cycle arrest, and apoptosis induced by chloroquine in human breast cancer cell line Bcap-37. | |
| | |
MedLine Citation:
|
PMID: 19088425 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Chloroquine is an antimalarial drug that has been used in the treatment and prophylaxis of malaria since the 1950s. The present study was undertaken to examine the effects of chloroquine on Bcap-37 human breast cancer cells' growth, cell cycle modulation, apoptosis induction, and associated molecular alterations in vitro. The chloroquine treatment decreased the viability of Bcap-37 cells in a concentration- and time-dependent manner, which correlated with G(2)/M phase cell cycle arrest. The chloroquine-mediated cell cycle arrest was associated with a decrease in protein levels/activity of polo-like kinase 1 (Plk1), phosphorylated cell division cycle 25C (Cdc25C), phosphorylated extracellular signal-regulated kinase 1/2 (ERK1/2), phosphorylated Akt. The chloroquine-treated Bcap-37 cells exhibited a marked decrease in the level of mitochondrial transmembrane potential (DeltaPsim), which was accompanied by the activation of caspase-3 and cleaved poly(ADP-ribose) polymerase (PARP). Exposure of Bcap-37 cells to chloroquine also resulted in the induction of spindle abnormalities. In conclusion, the findings in this study suggested that chloroquine might have potential anticancer efficacy, which could be attributed, in part, to its proliferation inhibition and apoptosis induction of cancer cells through modulation of apoptosis and cell cycle-related proteins expressions, down-regulation of mitochondrial transmembrane potential (DeltaPsim), and induction of spindle abnormalities. |
| | |
Authors:
|
Pei-du Jiang; Ying-lan Zhao; Wei Shi; Xiao-qiang Deng; Gang Xie; Yong-qiu Mao; Zheng-guang Li; Yu-zhu Zheng; Sheng-yong Yang; Yu-quan Wei |
Related Documents
:
|
12447975 - The dynamics of cell cycle regulation. 12938175 - Cell-cycle-regulatory elements and the control of cell differentiation in the budding y... 12829295 - The budding index of saccharomyces cerevisiae deletion strains identifies genes importa... 10559915 - Four-dimensional control of the cell cycle. 12908855 - Collagen gel contraction serves to rapidly distinguish epithelial- and mesenchymal-deri... 8955125 - The nuclear localization sequences of the brca1 protein interact with the importin-alph... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-12-09 |
Journal Detail:
|
Title: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology Volume: 22 ISSN: 1421-9778 ISO Abbreviation: Cell. Physiol. Biochem. Publication Date: 2008 |
Date Detail:
|
Created Date: 2008-12-17 Completed Date: 2009-02-10 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9113221 Medline TA: Cell Physiol Biochem Country: Switzerland |
Other Details:
|
Languages: eng Pagination: 431-40 Citation Subset: IM |
Copyright Information:
|
Copyright 2008 S. Karger AG, Basel. |
Affiliation:
|
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, Sichuan, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Apoptosis
/
drug effects* Breast Neoplasms / enzymology, pathology* Caspase 3 / metabolism Cell Cycle Proteins / metabolism Cell Division / drug effects* Cell Line, Tumor Cell Proliferation / drug effects Cell Shape / drug effects Chloroquine / pharmacology* Drug Screening Assays, Antitumor Enzyme Activation / drug effects G2 Phase / drug effects* Humans Membrane Potential, Mitochondrial / drug effects Mitotic Spindle Apparatus / drug effects |
| Chemical | |
Reg. No./Substance:
|
0/Cell Cycle Proteins; 54-05-7/Chloroquine; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Acute effects of haemodialysis on pro-/anti- apoptotic genes in peripheral blood leukocytes.
Next Document: Cell death induced by uridine 5'-triphosphate (UTP) in contrast to adenosine 5'-triphosphate (ATP) i...