Document Detail

Cell fusion studies in the Werner syndrome.
MedLine Citation:
PMID:  4083156     Owner:  NLM     Status:  MEDLINE    
The mechanism of retarded DNA synthesis was investigated by the cell fusion method. The [3H] TdR labeling index of young cells from patients with Werner syndrome (WS cells) X young normal human diploid fibroblast cells (NH cells) was considerably lower than that of NH homodikaryons, but was significantly higher than that of WS homodikaryons. The labeling index of old WS X young NH heterodikaryons was as low as that of WS homodikaryons. The labeling index of WS X HeLa heterodikaryons was the same as that of HeLa homodikaryons. These results indicate that WS cells are somewhat similar to senescent NH cells. The labeling indices in both WS karyoplast X NH heterodikaryons and WS cytoplast X NH cybrids were lower than those in normal karyoplast X NH heterodikaryons and normal cytoplast X NH cybrids, respectively. These results indicate that both nuclear and cytoplasmic environments are involved in the retarded DNA synthesis in WS cells. The most plausible interpretation of all our data is that the retarded DNA synthesis in WS cells could be caused by either the 'senescent factor(s)' or the deficiency of gene product(s) that is necessary for DNA synthesis and could be a secondary consequence of the genetic defect.
K Tanaka; K Yamamura; K Fukuchi; K Kawai; Y Kumahara
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Advances in experimental medicine and biology     Volume:  190     ISSN:  0065-2598     ISO Abbreviation:  Adv. Exp. Med. Biol.     Publication Date:  1985  
Date Detail:
Created Date:  1986-02-20     Completed Date:  1986-02-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0121103     Medline TA:  Adv Exp Med Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  341-51     Citation Subset:  IM    
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MeSH Terms
Cell Fusion
Cell Survival
Cells, Cultured
DNA / biosynthesis*
Fibroblasts / metabolism
Hela Cells
Thymidine / metabolism
Tritium / diagnostic use
Werner Syndrome / metabolism*,  pathology
Reg. No./Substance:
10028-17-8/Tritium; 50-89-5/Thymidine; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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