Document Detail


Cell fusion-induced reprogramming.
MedLine Citation:
PMID:  20336523     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Genomic reprogramming can be accomplished by five different types of methods: nuclear transfer, cell fusion, in vitro culture, introduction of egg extract, and transduction of transcription factors. We have shown that fusion-induced reprogramming is an efficient method for reprogramming differentiated somatic cells to a pluripotential state (pluripotential reprogramming)--Oct4 gene reactivation occurs within 1-2 days postfusion of somatic cells with pluripotent stem cells. Reactivation of Oct4 can be monitored by detection of the GFP signal from the Oct4-GFP transgene of somatic cells. In the current report, we fused double transgenic (OG2/ROSA26) somatic cells with pluripotent embryonic stem (ES) cells, and demonstrated the presence of the somatic cell genome in all GFP-positive ES-like colony-forming cells, confirming their identity as the cell fusion hybrids.
Authors:
Jeong Tae Do; Hans R Schöler
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  636     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-03-25     Completed Date:  2010-06-07     Revised Date:  2010-09-29    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  179-90     Citation Subset:  IM    
Affiliation:
CHA Stem Cell Institute & CHA Biotech, Pochon CHA University, Seoul, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation / physiology*
Cell Fusion / methods*
Cells, Cultured
Female
Fibroblasts / cytology,  physiology
Homeodomain Proteins
Male
Mice
Mice, Transgenic
Nuclear Reprogramming*
Octamer Transcription Factor-3 / genetics,  metabolism
Pluripotent Stem Cells / cytology,  physiology*
Proteins / genetics,  metabolism
Recombinant Fusion Proteins / genetics,  metabolism
Transcription Factors
Chemical
Reg. No./Substance:
0/Gt(ROSA)26Sor protein, mouse; 0/Homeodomain Proteins; 0/Octamer Transcription Factor-3; 0/Og2x protein, mouse; 0/Proteins; 0/Recombinant Fusion Proteins; 0/Transcription Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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