| Cell fusion assays for yeast mating pairs. | |
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MedLine Citation:
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PMID: 18979244 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Yeast mating provides an accessible genetic system for the discovery of fundamental mechanisms in eukaryotic cell fusion. Although aspects of yeast mating related to pheromone signaling and polarized growth have been intensively investigated, fusion itself is poorly understood. This chapter describes methods for measuring the overall efficiency of yeast cell fusion and for monitoring various stages of the fusion process including cell wall remodeling, plasma membrane fusion, and nuclear fusion. |
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Authors:
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Eric Grote |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 475 ISSN: 1064-3745 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2008 |
Date Detail:
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Created Date: 2008-11-03 Completed Date: 2008-11-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 165-96 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cytological Techniques
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methods* Genetic Complementation Test Luminescent Proteins / metabolism Pyridinium Compounds / metabolism Quaternary Ammonium Compounds / metabolism Reproduction Saccharomyces cerevisiae / cytology*, growth & development, physiology* Vacuoles / metabolism |
| Chemical | |
Reg. No./Substance:
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0/FM 4-64; 0/Luminescent Proteins; 0/Pyridinium Compounds; 0/Quaternary Ammonium Compounds |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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