Document Detail


Cell fusion assays for yeast mating pairs.
MedLine Citation:
PMID:  18979244     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Yeast mating provides an accessible genetic system for the discovery of fundamental mechanisms in eukaryotic cell fusion. Although aspects of yeast mating related to pheromone signaling and polarized growth have been intensively investigated, fusion itself is poorly understood. This chapter describes methods for measuring the overall efficiency of yeast cell fusion and for monitoring various stages of the fusion process including cell wall remodeling, plasma membrane fusion, and nuclear fusion.
Authors:
Eric Grote
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  475     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2008  
Date Detail:
Created Date:  2008-11-03     Completed Date:  2008-11-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  165-96     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.
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MeSH Terms
Descriptor/Qualifier:
Cytological Techniques / methods*
Genetic Complementation Test
Luminescent Proteins / metabolism
Pyridinium Compounds / metabolism
Quaternary Ammonium Compounds / metabolism
Reproduction
Saccharomyces cerevisiae / cytology*,  growth & development,  physiology*
Vacuoles / metabolism
Chemical
Reg. No./Substance:
0/FM 4-64; 0/Luminescent Proteins; 0/Pyridinium Compounds; 0/Quaternary Ammonium Compounds

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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