Document Detail


Cell fusion assays for yeast mating pairs.
MedLine Citation:
PMID:  18979244     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Yeast mating provides an accessible genetic system for the discovery of fundamental mechanisms in eukaryotic cell fusion. Although aspects of yeast mating related to pheromone signaling and polarized growth have been intensively investigated, fusion itself is poorly understood. This chapter describes methods for measuring the overall efficiency of yeast cell fusion and for monitoring various stages of the fusion process including cell wall remodeling, plasma membrane fusion, and nuclear fusion.
Authors:
Eric Grote
Related Documents :
15121224 - A dendritic cell based hybrid cell vaccine generated by electrofusion for immunotherapy...
21863174 - [60]fullerene-based monolayers as neuroprotective biocompatible hybrid materials.
15367644 - Cell fusion activities of hantaan virus envelope glycoproteins.
194844 - Differential induction of epstein-barr virus-related antigens in heterokaryon cultures.
21940394 - Evaluation of correct endogenous reactive oxygen species content for human sperm capaci...
20963234 - Electrical cell-substrate impedance sensing as a non-invasive tool for cancer cell study.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  475     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2008  
Date Detail:
Created Date:  2008-11-03     Completed Date:  2008-11-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  165-96     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cytological Techniques / methods*
Genetic Complementation Test
Luminescent Proteins / metabolism
Pyridinium Compounds / metabolism
Quaternary Ammonium Compounds / metabolism
Reproduction
Saccharomyces cerevisiae / cytology*,  growth & development,  physiology*
Vacuoles / metabolism
Chemical
Reg. No./Substance:
0/FM 4-64; 0/Luminescent Proteins; 0/Pyridinium Compounds; 0/Quaternary Ammonium Compounds

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Macrophage fusion: molecular mechanisms.
Next Document:  Ultrastructural analysis of cell fusion in yeast.