Document Detail

Cell-free synthesis of mycolic acids in Mycobacterium aurum: radioactivity distribution in newly synthesized acids and presence of cell wall in the system.
MedLine Citation:
PMID:  2196087     Owner:  NLM     Status:  MEDLINE    
Distribution of radiolabelling in different parts of the newly synthesized mycolic acids, by a cell-free system from Mycobacterium aurum previously described, is examined, [1-14C]acetate being the precursor. By oxidation cleavage of mycolic acids and examination of the fragments, it was shown that acetate was not uniformly incorporated into the molecule: the methyl terminal part was not labelled, while the central fragments--between unsaturations or between oxygenated functions (oxo or ester) and unsaturations--presented the major part of radioactivity, suggesting the elongation of a preformed compound that the cell-free extract was unable to synthesize. Moreover, the side-chain R2-CH2-COOH was only weakly labelled compared to the central fragments. Since non-hydroxylated fatty acids were not synthesized by the system, it is suggested that de novo C18 fatty acids may be elongated with C2 units by the cell-free extract into C22 fatty derivative, only a low level of labelling being recorded (two C2 units for all the molecule). A scheme is proposed to summarize the main results. Identification of meso-DAP which is a characteristic amino-acid of the peptidoglycan in Actinomycetes and analysis of the profiles of total fatty esters, demonstrated that the cell-free extract is partly constituted by fragments of the cell wall as has already been noticed by examination of micrographs of the extract.
C Lacave; A Quémard; G Lanéelle
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1045     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1990 Jun 
Date Detail:
Created Date:  1990-08-23     Completed Date:  1990-08-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  58-68     Citation Subset:  IM    
Centre de Recherche de Biochimie et de Génétique Cellulaires, C.N.R.S., Université Paul Sabatier, Toulouse, France.
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MeSH Terms
Acetates / metabolism
Carbon Radioisotopes
Cell Wall / metabolism
Cell-Free System
Chromatography, Gas
Mycobacterium / metabolism*
Mycolic Acids / isolation & purification,  metabolism*
Radioisotope Dilution Technique
Reg. No./Substance:
0/Acetates; 0/Carbon Radioisotopes; 0/Mycolic Acids

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