| Cell-free fetal nucleic acids in amniotic fluid. | |
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MedLine Citation:
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PMID: 20923874 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Research into cell-free fetal (cff) nucleic acids has primarily focused on maternal plasma; however, cff DNA and RNA are also detectable in other body fluids such as amniotic fluid (AF). In AF, cff DNA is present in much greater concentrations than in maternal plasma and represents a pure fetal sample uncontaminated by maternal- and trophoblast-derived nucleic acids. The aim of this review was to summarize the current knowledge on cff nucleic acids in AF and to outline future research directions. METHODS: MEDLINE and PREMEDLINE were searched up to August 2010 for original investigations of cell-free RNA or DNA in AF. Sixteen studies were included in the review. RESULTS: AF cff DNA represents a physiologically separate pool from cff DNA in maternal plasma. The placenta is not a major source of nucleic acids in AF. It is feasible to isolate cff nucleic acids from small volumes of discarded AF supernatant in sufficient quality and quantity to perform microarray studies and downstream applications such as pathway analysis. This 'discovery-driven approach' has resulted in new information on the pathogenesis of Down syndrome and polyhydramnios. There is otherwise a paucity of information relating to the basic biology and clinical applications of cff nucleic acids in AF. CONCLUSIONS: AF supernatant is a valuable and widely available but under-utilized biological resource. Further studies of cff nucleic acids in AF may lead to new insights into human fetal development and ultimately new approaches to antenatal treatment of human disease. |
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Authors:
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L Hui; D W Bianchi |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review Date: 2010-10-05 |
Journal Detail:
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Title: Human reproduction update Volume: 17 ISSN: 1460-2369 ISO Abbreviation: Hum. Reprod. Update Publication Date: 2011 May-Jun |
Date Detail:
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Created Date: 2011-04-08 Completed Date: 2011-08-12 Revised Date: 2012-05-01 |
Medline Journal Info:
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Nlm Unique ID: 9507614 Medline TA: Hum Reprod Update Country: England |
Other Details:
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Languages: eng Pagination: 362-71 Citation Subset: IM |
Affiliation:
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Mother Infant Research Institute at Tufts Medical Center, Boston, MA 02111, USA. lhui@tuftsmedicalcenter.org |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amniotic Fluid
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chemistry* DNA / analysis* Female Fetus* Humans Maternal-Fetal Exchange Pregnancy Prenatal Diagnosis Proteome RNA / analysis* |
| Grant Support | |
ID/Acronym/Agency:
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HD 42053-07/HD/NICHD NIH HHS; R01 HD042053-09/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Proteome; 63231-63-0/RNA; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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