Document Detail

Cell-free extract(s) of Pseudomonas putida catalyzes the conversion of cyanides, cyanates, thiocyanates, formamide, and cyanide-containing mine waters into ammonia.
MedLine Citation:
PMID:  8920201     Owner:  NLM     Status:  MEDLINE    
Our isolate, Pseudomonas putida, is known to be capable of utilizing cyanides as the sole source of carbon (C) and nitrogen (N) both in the form of free cells and cells immobilized in calcium alginate. In the present study, the cell-free extract(s) were prepared from the cells of P. putida grown in the presence of sodium cyanide. The ability of enzyme(s) to convert cyanides, cyanates, thiocyanates, formamide and cyanide-containing mine waters into ammonia (NH3) was studied at pH 7.5 and pH 9.5. The kinetic analysis of cyanide and formamide conversion into NH3 at pH 7.5 and pH 9.5 by the cell-free extract(s) of P. putida was also studied. The Km and Vmax values for cyanide/formamide were found to be 4.3/8 mM and 142/227 mumol NH3 released mg protein-1 min-1 respectively at pH 7.5 and 5/16.67 mM and 181/434 mumol NH3 released mg protein-1 h-1 respectively at pH 9.5. The study thus concludes that the cell-free extract(s) of P. putida is able to metabolize not only cyanides, cyanates, thiocyanates, and formamide but also cyanide-containing mine waters to NH3.
G R Babu; O K Vijaya; V L Ross; J H Wolfram; K D Chapatwala
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  45     ISSN:  0175-7598     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  1996 Mar 
Date Detail:
Created Date:  1997-01-02     Completed Date:  1997-01-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  273-7     Citation Subset:  B    
Division of Natural Sciences, Selma University, AL 36701, USA.
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MeSH Terms
Ammonia / metabolism
Biodegradation, Environmental
Cyanates / metabolism*
Cyanides / metabolism*
Formamides / metabolism*
Hydrogen-Ion Concentration
Pseudomonas putida / enzymology,  metabolism*
Thiocyanates / metabolism*
Water Pollutants, Chemical / metabolism*
Reg. No./Substance:
0/Cyanates; 0/Cyanides; 0/Formamides; 0/Thiocyanates; 0/Water Pollutants, Chemical; 75-12-7/formamide; 7664-41-7/Ammonia

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