Document Detail


Cell enrichment and immunochemical staining.
MedLine Citation:
PMID:  15596905     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell populations can be enriched using a number of physicochemical methods based on characteristics, such as density, size, and volume. Centrifugation using density gradients is typically applied to isolate populations with similar characteristics. Cells obtained using these methods can be further authenticated using specific antibodies that react to markers on the cell surface, allowing the identification of cell subtypes.
Authors:
Oscar R Lara; Jeffrey J Chalmers
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  295     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2005  
Date Detail:
Created Date:  2004-12-14     Completed Date:  2005-05-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  301-10     Citation Subset:  IM    
Affiliation:
Department of Chemical Engineering, The Ohio State University, Columbus, OH, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies / chemistry*
Antigens, Surface / chemistry*
Cell Separation / methods*
Centrifugation, Density Gradient / methods
Immunohistochemistry / methods*
Chemical
Reg. No./Substance:
0/Antibodies; 0/Antigens, Surface

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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