Document Detail


Cell encapsulation within PVA-based hydrogels via freeze-thawing: a one-step scaffold formation and cell storage technique.
MedLine Citation:
PMID:  19598204     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cryogelation is a physical hydrogel formation method for certain polymers, notably polyvinyl alcohol (PVA). The hypothesis of this study is that a PVA-based solution with the necessary intracellular cryoprotectant and nutrient supply can be used, first for storage of vascular smooth muscle cells, and subsequently to form a suitable tissue-engineering scaffold during the thawing process. Bovine arterial smooth muscle cells were encapsulated within PVA-gelatin hydrogels over a wide range of serum, DMSO and cell culture medium concentrations. Several parameters expected to affect gelation and cell viability (PVA viscosity, DMSO concentration, serum presence) were assessed with experimental designs and the optimal conditions for cell survival were determined. Cell viability can be improved by increasing concentration of DMSO and serum without compromising the gelation process. An additional crosslinking step using a coagulation bath was beneficial for hydrogel stability but caused peripheral accumulation of cells. In conclusion, a freeze-thaw process can be utilized to prepare and store cell-laden hydrogels with adjustable mechanical properties.
Authors:
N E Vrana; A O'Grady; E Kay; P A Cahill; G B McGuinness
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of tissue engineering and regenerative medicine     Volume:  3     ISSN:  1932-7005     ISO Abbreviation:  J Tissue Eng Regen Med     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-09-30     Completed Date:  2010-02-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101308490     Medline TA:  J Tissue Eng Regen Med     Country:  England    
Other Details:
Languages:  eng     Pagination:  567-72     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2009 John Wiley & Sons, Ltd.
Affiliation:
Dublin City University, Materials Processing Research Centre, Glasnevin, Dublin 9, Ireland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Cell Culture Techniques / instrumentation,  methods*
Cell Survival
Cross-Linking Reagents / chemistry
Culture Media / chemistry
Dimethyl Sulfoxide / chemistry
Freezing
Hydrogels / chemistry*
Microscopy, Electron, Scanning / methods
Muscle, Smooth, Vascular / cytology
Polyvinyl Alcohol / chemistry*
Specimen Handling
Thoracic Arteries / cytology
Tissue Engineering / instrumentation*,  methods
Chemical
Reg. No./Substance:
0/Cross-Linking Reagents; 0/Culture Media; 0/Hydrogels; 0/polyvinyl alcohol hydrogel; 67-68-5/Dimethyl Sulfoxide; 9002-89-5/Polyvinyl Alcohol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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