| Cell encapsulation and oxygenation in nanoporous microcontainers. | |
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MedLine Citation:
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PMID: 19629700 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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With strides in stem cell biology, cell engineering and molecular therapy, the transplantation of cells to produce therapeutic molecules endogenously is an attractive and achievable alternative to the use of exogenous drugs. The encapsulation of such cell transplants in semi-permeable, nanoporous constructs is often required to protect them from immune attack and to prevent their proliferation in the host. However, effective graft immunoisolation has been mostly elusive owing to the absence of a high-throughput method to create precisely controlled, high-aspect-ratio nanopores. To address the clinical need for effective cell encapsulation and immunoisolation, we devised a biocompatible cell-encapsulating microcontainer and a method to create highly anisotropic nanopores in the microcontainer's surface. To evaluate the efficacy of these nanopores in oxygenating the encapsulated cells, we engineered 9L rat glioma cells to bioluminesce under hypoxic conditions. The methods described above should aid in evaluating the long term survival and efficacy of cellular grafts. |
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Authors:
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Barjor Gimi; Joonbum Kwon; Li Liu; Yang Su; Krishnamurthy Nemani; Krutarth Trivedi; Yonghao Cui; Behroze Vachha; Ralph Mason; Wenchuang Hu; Jeong-Bong Lee |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biomedical microdevices Volume: 11 ISSN: 1572-8781 ISO Abbreviation: Biomed Microdevices Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-11-12 Completed Date: 2010-01-20 Revised Date: 2011-09-21 |
Medline Journal Info:
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Nlm Unique ID: 100887374 Medline TA: Biomed Microdevices Country: United States |
Other Details:
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Languages: eng Pagination: 1205-12 Citation Subset: IM |
Affiliation:
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UT Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX 75390, USA. barjorg@yahoo.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Transplantation / methods* Cells, Cultured Oxygen / metabolism* Porosity Rats |
| Grant Support | |
ID/Acronym/Agency:
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R01 EB007456/EB/NIBIB NIH HHS; R01 EB007456-03/EB/NIBIB NIH HHS; S10 RR024757-01/RR/NCRR NIH HHS; SIO RR024757/RR/NCRR NIH HHS; U24 CA 126608/CA/NCI NIH HHS; U24 CA126608-01/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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7782-44-7/Oxygen |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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