Document Detail


Cell divisions are required for L1 retrotransposition.
MedLine Citation:
PMID:  17145770     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
LINE-1 (L1) retrotransposons comprise a large fraction of genomic DNAs of many organisms. Many L1 elements are active and may generate potentially deleterious mutations by inserting into genes, yet little is known about the control of retrotransposition by the host. Here we examined whether retrotransposition depends on the cell cycle by using a retrotransposition assay with cultured human cells. We show that in both cancer cells and primary human fibroblasts, retrotransposition was strongly inhibited in the cells arrested in the G(1), S, G(2), or M stage of the cell cycle. Retrotransposition was also inhibited during cellular senescence in primary human fibroblasts. The levels of L1 transcripts were strongly reduced in arrested cells, suggesting that the reduction in L1 transcript abundance limits retrotransposition in nondividing cells. We hypothesize that inhibition of retrotransposition in nondividing cells protects somatic tissues from accumulation of deleterious mutations caused by L1 elements.
Authors:
Xi Shi; Andrei Seluanov; Vera Gorbunova
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-12-04
Journal Detail:
Title:  Molecular and cellular biology     Volume:  27     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-01-31     Completed Date:  2007-03-16     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1264-70     Citation Subset:  IM    
Affiliation:
University of Rochester, 213 Hutchison Hall, River Campus, Rochester, NY 14627-0211, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Aging
Cell Division*
Cells, Cultured
Fibroblasts / cytology*
Gene Expression Regulation
HeLa Cells
Humans
Long Interspersed Nucleotide Elements / genetics*
RNA, Messenger / genetics,  metabolism
Chemical
Reg. No./Substance:
0/RNA, Messenger
Comments/Corrections

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