Document Detail


Cell division induced by mechanical stimulation in starved Tetrahymena thermophila: cell cycle without synthesis of macronuclear DNA.
MedLine Citation:
PMID:  15261157     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Starved Tetrahymena thermophila cells underwent synchronous cell division 2 h after a mechanical stimulation. The macronucleus showed no obvious increase in DNA content before the cell division in the starvation medium, and the DNA content was decreased after the cell division. On the other hand, when the starved cells were given nutrient-supplied medium immediately after the mechanical stimulation, cell division was delayed for 3 h. This period was almost the same as that for G1 cells in the stationary culture to first division after transfer to fresh nutrient medium. These results suggest that the mechanical stimulation induces an early division of starved cells, skipping the macronuclear S-phase with the starved cells probably becoming trapped in G1. Starved cells that had finished division soon formed mating pairs with cells of the opposite type. These observations lead us to propose that cell division in starvation conditions may be necessary to reduce macronuclear DNA content prior to the mating of T. thermophila.
Authors:
Masaaki Iwamoto; Toshiro Sugai; Yasuo Nakaoka
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell biology international     Volume:  28     ISSN:  1065-6995     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2004  
Date Detail:
Created Date:  2004-07-20     Completed Date:  2005-07-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  503-9     Citation Subset:  IM    
Affiliation:
Division of Biophysical Engineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-853, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle / physiology*
Cell Division*
Centrifugation
Culture Media / chemistry
DNA Replication*
Macronucleus / genetics*
Stress, Mechanical
Tetrahymena thermophila / cytology,  physiology*
Chemical
Reg. No./Substance:
0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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