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Cell death and neuronal differentiation of glioblastoma stem-like cells induced by neurogenic transcription factors.
MedLine Citation:
PMID:  23047160     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Glioblastoma multiform (GBM) are devastating brain tumors containing a fraction of multipotent stem-like cells which are highly tumorigenic. These cells are resistant to treatments and are likely to be responsible for tumor recurrence. One approach to eliminate GBM stem-like cells would be to force their terminal differentiation. During development, neurons formation is controlled by neurogenic transcription factors such as Ngn1/2 and NeuroD1. We found that in comparison with oligodendrogenic genes, the expression of these neurogenic genes is low or absent in GBM tumors and derived cultures. We thus explored the effect of overexpressing these neurogenic genes in three CD133(+) Sox2(+) GBM stem-like cell cultures and the U87 glioma line. Introduction of Ngn2 in CD133(+) cultures induced massive cell death, proliferation arrest and a drastic reduction of neurosphere formation. Similar effects were observed with NeuroD1. Importantly, Ngn2 effects were accompanied by the downregulation of Olig2, Myc, Shh and upregulation of Dcx and NeuroD1 expression. The few surviving cells adopted a typical neuronal morphology and some of them generated action potentials. These cells appeared to be produced at the expense of GFAP(+) cells which were radically reduced after differentiation with Ngn2. In vivo, Ngn2-expressing cells were unable to form orthotopic tumors. In the U87 glioma line, Ngn2 could not induce neuronal differentiation although proliferation in vitro and tumoral growth in vivo were strongly reduced. By inducing cell death, cell cycle arrest or differentiation, this work supports further exploration of neurogenic proteins to oppose GBM stem-like and non-stem-like cell growth. © 2012 Wiley Periodicals, Inc.
Authors:
Pierre-Olivier Guichet; Ivan Bieche; Marisa Teigell; Ché Serguera; Bernard Rothhut; Valérie Rigau; Frédérique Scamps; Chantal Ripoll; Sophie Vacher; Sylvie Taviaux; Hugues Chevassus; Hugues Duffau; Jacques Mallet; Aurélie Susini; Dominique Joubert; Luc Bauchet; Jean-Philippe Hugnot
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-9
Journal Detail:
Title:  Glia     Volume:  -     ISSN:  1098-1136     ISO Abbreviation:  Glia     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8806785     Medline TA:  Glia     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Affiliation:
INSERM U1051, Institut des Neurosciences de Montpellier, Hôpital St Eloi, 80 av Augustin, Fliche 34091, Montpellier, Cedex 05, France. pierre-olivier.guichet@inserm.fr.
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