| Cell death-inducing DFF45-like effector b is present in pancreatic beta-cells and involved in palmitate induced beta-cell apoptosis. | |
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MedLine Citation:
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PMID: 21948526 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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BACKGROUND: Excessive accumulation of long-chain fatty acids in the pancreatic islets is associated with beta cell dysfunction and ultimately contributes to the pathogenesis of type 2 diabetes. It has been well proved that the cell death-inducing DFF45-like effector b (Cideb) is involved in cell apoptosis and lipid metabolism. However, the expression and function of Cideb in endocrine pancreas remain to be investigated. METHODS: By using reverse-transcript PCR, immunohistochemistry and Western blot, we observed the expression of Cideb in pancreas tissues and clonal beta-cell lines. The physiological role of Cideb was examined under the free fatty acid (FFA) administration and Cideb RNA interference, and further analysis on apoptosis was measured by TUNEL assay and caspase-3 activity. Nile red staining and quantitative evaluation of triglyceride were used to detect the lipid accumulation. The changes in esterification of FFA were traced by radiolabeled palmitate. RESULTS: Cideb was abundantly expressed in pancreas, and mainly localized in beta cells. FFAs, especially palmitate, induced an obvious increase of Cideb expression in beta cell lines. Adenoviral-mediated overexpression of Cideb increased the apoptosis, whereas RNA interference-based Cideb depletion in beta-TC3 cells had no effect on apoptosis in normal condition. Palmitate supplementation led to beta cell lipo-apoptosis, and Cideb silencing exacerbated the apoptosis induced by palmitate, reduced intracellular triglyceride content and aggravated FFA overload in beta cells. CONCLUSIONS: The present results suggest that increased Cideb expression upon palmitate stimulus may be involved in beta cell lipo-apoptosis though influencing conversion of FFAs to lipid esters in lipid droplets. Copyright © 2011 John Wiley & Sons, Ltd. |
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Authors:
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H Li; Y Song; Lj Zhang; Ff Li; Y Gu; J Zhang; Wp Dong; L Xue; Ly Zhang; F Liu; J Wang; Ln Jiang; J Ye; Q Li |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-21 |
Journal Detail:
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Title: Diabetes/metabolism research and reviews Volume: - ISSN: 1520-7560 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-9-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100883450 Medline TA: Diabetes Metab Res Rev Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 John Wiley & Sons, Ltd. |
Affiliation:
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State Key Laboratory of Cancer Biology and Department of Pathology, Xijing Hospital, Fourth Military Medical University, CN-710032, Xi'an, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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