Document Detail


Cell death in mammalian cell culture: molecular mechanisms and cell line engineering strategies.
MedLine Citation:
PMID:  20502964     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Cell death is a fundamentally important problem in cell lines used by the biopharmaceutical industry. Environmental stress, which can result from nutrient depletion, by-product accumulation and chemical agents, activates through signalling cascades regulators that promote death. The best known key regulators of death process are the Bcl-2 family proteins which constitute a critical intracellular checkpoint of apoptosis cell death within a common death pathway. Engineering of several members of the anti-apoptosis Bcl-2 family genes in several cell types has extended the knowledge of their molecular function and interaction with other proteins, and their regulation of cell death. In this review, we describe the various modes of cell death and their death pathways at molecular and organelle level and discuss the relevance of the growing knowledge of anti-apoptotic engineering strategies to inhibit cell death and increase productivity in mammalian cell culture.
Authors:
Britta Krampe; Mohamed Al-Rubeai
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Publication Detail:
Type:  Journal Article     Date:  2010-05-26
Journal Detail:
Title:  Cytotechnology     Volume:  62     ISSN:  0920-9069     ISO Abbreviation:  Cytotechnology     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-09-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8807027     Medline TA:  Cytotechnology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  175-88     Citation Subset:  B    
Affiliation:
School of Chemical and Bioprocess Engineering, and Conway Institute of Biomolecular & Biomedical Research, University College Dublin, Belfield, Dublin 4, Republic of Ireland.
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