Document Detail


Cell death of human oral squamous cell carcinoma cell line induced by herpes simplex virus thymidine kinase gene and ganciclovir.
MedLine Citation:
PMID:  14727689     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Suicide gene therapy combining herpes simplex virus thymidine kinase gene (HSVtk) and ganciclovir (GCV) is one strategy for the treatment of head and neck squamous cell carcinoma (HNSCC). The purpose of this study is to determine the mechanism of cell death that occurs in suicide gene therapy using HSVtk and GCV and to assess the safety of that therapy. The human oral squamous cell carcinoma cell line SAS was treated with adenovirus vector containing HSVtk gene (AdHSVtk) and GCV in vitro. Morphological changes including chromatin condensation, cell shrinkage, blebbing of cell membrane, and ballooning formations were observed. Changes in the localization of phospholipids in the cell membrane were also observed. The results of flow cytometry showed a maximum of about 65% of cells in the early phase of apoptosis. In addition, DNA fragmentation was investigated using the TUNEL method in vivo. Nude mice (BALB/c AJD(-nu-), aged 4 weeks) were implanted with SAS and treated with AdHSVtk and GCV. Tumor sections were then observed. The treatment group was confirmed to have DNA fragmentation-positive cells. These results suggest that suicide gene therapy using AdHSVtk and GCV led to apoptosis of the oral squamous cell carcinoma cell line.
Authors:
Masaya Nishikawa; Yasushi Hayashi; Noriyuki Yamamoto; Takafumi Fukui; Hirokazu Fukuhara; Kenji Mitsudo; Iwai Tohnai; Minoru Ueda; Masaaki Mizuno; Jun Yoshida
Related Documents :
2721359 - Identification of features of metaplastic cells in sputum for the detection of squamous...
10211549 - Modulating effect of resveratrol and quercetin on oral cancer cell growth and prolifera...
1998989 - Measurement of in vitro cellular pharmacokinetics of 5-fluorouracil in human and rat ca...
1384369 - Basaloid squamous cell carcinoma of the head and neck. a clinicopathologic and immunohi...
18691699 - Establishment of a highly metastatic tongue squamous cell carcinoma cell line from new ...
20972659 - Part i. development of a model system for studying nitric oxide in tumors: high nitric ...
23464429 - Senescence as a consequence of ginsenoside rg1 response on k562 human leukemia cell line.
20607619 - Feeder-independent continuous culture of the picm-19 pig liver stem cell line.
16417739 - Poor development of human nuclear transfer embryos using failed fertilized oocytes.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nagoya journal of medical science     Volume:  66     ISSN:  0027-7622     ISO Abbreviation:  Nagoya J Med Sci     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2004-01-19     Completed Date:  2004-02-27     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0412011     Medline TA:  Nagoya J Med Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  129-37     Citation Subset:  IM    
Affiliation:
Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adenoviridae / genetics
Animals
Apoptosis*
Carcinoma, Squamous Cell / pathology*
Cell Line, Tumor
Cell Membrane / metabolism
Cell Separation
Chromatin / metabolism
DNA Fragmentation
Flow Cytometry
Ganciclovir / therapeutic use*
Humans
In Situ Nick-End Labeling
Lipid Bilayers / metabolism
Mice
Mice, Inbred BALB C
Mice, Nude
Microscopy, Fluorescence
Mouth Neoplasms / pathology*
Simplexvirus / enzymology*
Thymidine Kinase / genetics*
Chemical
Reg. No./Substance:
0/Chromatin; 0/Lipid Bilayers; 82410-32-0/Ganciclovir; EC 2.7.1.21/Thymidine Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Radiation therapy for metastatic brain tumors from lung cancer--a review to devise individualized tr...
Next Document:  Inception rates of deliberate self-harm among adolescents in West London.