Document Detail


Cell death goes LIVE: technological advances in real-time tracking of cell death.
MedLine Citation:
PMID:  20519963     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell population can be viewed as a quantum system, which like Schrödinger's cat exists as a combination of survival- and death-allowing states. Tracking and understanding cell-to-cell variability in processes of high spatio-temporal complexity such as cell death is at the core of current systems biology approaches. As probabilistic modeling tools attempt to impute information inaccessible by current experimental approaches, advances in technologies for single-cell imaging and omics (proteomics, genomics, metabolomics) should go hand in hand with the computational efforts. Over the last few years we have made exciting technological advances that allow studies of cell death dynamically in real-time and with the unprecedented accuracy. These approaches are based on innovative fluorescent assays and recombinant proteins, bioelectrical properties of cells, and more recently also on state-of-the-art optical spectroscopy. Here, we review current status of the most innovative analytical technologies for dynamic tracking of cell death, and address the interdisciplinary promises and future challenges of these methods.
Authors:
Joanna Skommer; Zbigniew Darzynkiewicz; Donald Wlodkowic
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Publication Detail:
Type:  Journal Article; Review     Date:  2010-06-15
Journal Detail:
Title:  Cell cycle (Georgetown, Tex.)     Volume:  9     ISSN:  1551-4005     ISO Abbreviation:  Cell Cycle     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2011-05-17     Completed Date:  2011-11-23     Revised Date:  2014-09-11    
Medline Journal Info:
Nlm Unique ID:  101137841     Medline TA:  Cell Cycle     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2330-41     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Caspases / metabolism
Cell Communication
Cell Death
Cell Membrane / metabolism
Humans
Models, Biological*
Systems Biology*
Grant Support
ID/Acronym/Agency:
R01 CA028704/CA/NCI NIH HHS; R01 CA028704-30/CA/NCI NIH HHS; R01 CA028704-31/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
EC 3.4.22.-/Caspases
Comments/Corrections

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