Document Detail


Cell death and CD14 expression in resident and inflammatory polymorphonuclear leukocytes from virgin bovine mammary gland.
MedLine Citation:
PMID:  20599238     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
This paper investigates the association between expression of CD14 and occurrence of apoptosis in blood, resident ((RES)PMN) and inflammatory ((INF)PMN) polymorphonuclear leukocytes (PMN) from heifer mammary glands. The fresh population of (RES)PMN contained a statistically significant higher proportion of CD14+, apoptotic and necrotic cells than did populations of (INF)PMN and blood PMN. In vitro cultivation of (RES)PMN, (INF)PMN and blood PMN led to concurrent increase of apoptotic, necrotic and CD14+ cells. A positive correlation was found between the proportions of both apoptotic and necrotic PMN and CD14+ PMN as determined by three-color flow cytometry analysis. Our study confirmed that expression of CD14 in blood PMN, (RES)PMN and (INF)PMN from heifer mammary glands was accompanied by apoptosis and necrosis.
Authors:
Zbysek Sladek; Dusan Rysanek
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Research in veterinary science     Volume:  90     ISSN:  1532-2661     ISO Abbreviation:  Res. Vet. Sci.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-02-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401300     Medline TA:  Res Vet Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  226-34     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Morphology, Physiology and Veterinary Sciences, Mendel University of Agriculture and Forestry, Zemedelska 1, Brno 613 00, Czech Republic.
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