| Cell cycling and differentiation do not require the retinoblastoma protein during early Xenopus development. | |
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MedLine Citation:
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PMID: 17188261 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The retinoblastoma protein (pRb) is a central regulator of the cell cycle, controlling passage through G1 phase. Moreover, pRb has also been shown to play a direct role in the differentiation of multiple tissues, including nerve and muscle. Rb null mice display embryonic lethality, although recent data have indicated that at least some of these defects are due to placental insufficiency. To investigate this further, we have examined the role of pRb in early development of the frog Xenopus laevis, which develops without the need for a placenta. Surprisingly, we see that loss of pXRb has no effect on either cell cycling or differentiation of neural or muscle tissue, while overexpression of pXRb similarly has no effects. We demonstrate that, in fact, pXRb is maintained in a hyperphosphorylated and therefore inactive state early in development. Therefore, Rb protein is not required for cell cycle control or differentiation in early embryos, indicating unusual control of these G1/G0 events at this developmental stage. |
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Authors:
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Ruth A Cosgrove; A Philpott |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2006-11-11 |
Journal Detail:
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Title: Developmental biology Volume: 303 ISSN: 0012-1606 ISO Abbreviation: Dev. Biol. Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-02-19 Completed Date: 2007-04-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372762 Medline TA: Dev Biol Country: United States |
Other Details:
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Languages: eng Pagination: 311-24 Citation Subset: IM |
Affiliation:
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Department of Oncology, University of Cambridge, Hutchison/MRC Research Centre, Addenbrookes Hospital, Hills Road, Cambridge CB2 0XZ, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western Bromodeoxyuridine Cell Cycle / physiology* Cell Differentiation / physiology* Embryonic Development / physiology* Immunohistochemistry In Situ Hybridization In Situ Nick-End Labeling Oligonucleotides Retinoblastoma Protein / metabolism* Xenopus laevis / embryology* |
| Chemical | |
Reg. No./Substance:
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0/Oligonucleotides; 0/Retinoblastoma Protein; 59-14-3/Bromodeoxyuridine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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