Document Detail

Cell cycle variation of Hsp70 levels in HeLa cells at 37 degrees C and after a heat shock.
MedLine Citation:
PMID:  7593215     Owner:  NLM     Status:  MEDLINE    
The expression of the 72 kD inducible heat shock protein (hsp72) has been reported to be cell cycle associated in unheated, synchronized HeLa cells. In this study, flow cytometry was used to investigate hsp72 levels through the cell cycle in HeLa cells by dual labeling with propidium iodide and antibodies against hsp72. The entire cell cycle distribution of hsp72 could be measured in a single sample of asynchronously growing cells. For unheated cells, the level of hsp72 increased about 30% from G1 to S phase, with about a 65% increase in G2/M, probably due to cell size differences. Neither mitotic selection nor serum stimulation induced a higher level of hsp72 than in the control cells. Western blot analysis of hsp72 from Hoechst-stained cells sorted from G1, mid-S, or G2/M showed that G1 cells had the lowest level of hsp72, with about a 30% increase in S phase and a 60% increase in G2/M, in good agreement with the flow cytometry results. These data conflict with previous reports by other laboratories which showed a 3-fold higher level of hsp72 in S phase than in G1 or G2. In contrast, heat shock (both acute and chronic) led to a non-uniform increase in hsp72 through the cell cycle. Most cells in mid S phase had high levels of hsp72, and a larger range in the levels of hsp72 were found in G1 and late S/G2/M phase cells.
H Hang; L He; M H Fox
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  165     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1995-12-05     Completed Date:  1995-12-05     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  367-75     Citation Subset:  IM    
Department of Radiological Health Sciences, Colorado State University, Fort Collins 80523, USA.
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MeSH Terms
Blotting, Western
Cell Cycle / physiology
DNA / metabolism
Flow Cytometry
HSP70 Heat-Shock Proteins / metabolism*
Hela Cells
Hot Temperature*
Shock / metabolism*,  pathology
Grant Support
Reg. No./Substance:
0/HSP70 Heat-Shock Proteins; 9007-49-2/DNA

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