| Cell-cycle synchrony for analysis of S. pombe DNA replication. | |
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MedLine Citation:
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PMID: 19563121 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Analysis of S phase events requires the ability to synchronize the cell cycle for subsequent physiological or molecular analysis. The cell cycle can be arrested at different stages, using drugs or temperature-sensitive mutations, and then released (block and release). These methods offer high levels of synchrony. Synchrony is lower in methods that fractionate the cells (lactose gradient synchrony), which avoids any cell-cycle perturbation. The degree of synchronization is assessed by monitoring cell morphology or DNA content. The choice of method is influenced by the needs of the experiment and the requirements of any mutations already in the strain of interest. |
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Authors:
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Douglas D Luche; Susan L Forsburg |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 521 ISSN: 1064-3745 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2009 |
Date Detail:
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Created Date: 2009-06-30 Completed Date: 2009-08-12 Revised Date: 2010-12-03 |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 437-48 Citation Subset: IM |
Affiliation:
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Department of Molecular and Computational Biology, University of Southern California, Los Angeles, CA, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle*
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drug effects Cell Cycle Proteins / genetics DNA Replication* DNA, Fungal / biosynthesis Genes, Fungal Hydroxyurea / pharmacology Lactose / metabolism Mutation Mycology / methods* Nitrogen / metabolism S Phase Schizosaccharomyces / cytology*, genetics, metabolism* Schizosaccharomyces pombe Proteins / genetics Staining and Labeling Temperature Transcription Factors / genetics |
| Grant Support | |
ID/Acronym/Agency:
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R01 GM059321-11/GM/NIGMS NIH HHS; R01 GM59321/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/DNA, Fungal; 0/Schizosaccharomyces pombe Proteins; 0/Transcription Factors; 0/cdc10 protein, S pombe; 127-07-1/Hydroxyurea; 63-42-3/Lactose; 7727-37-9/Nitrogen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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