Document Detail

Cell cycle synchronization of tobacco BY-2 cells.
MedLine Citation:
PMID:  17406517     Owner:  NLM     Status:  MEDLINE    
Synchronization is a powerful technique for understanding cell cycle events. Here, we describe the procedure for synchronizing tobacco bright yellow 2 (BY-2) cell line, with which an exceptionally high level of synchrony can be achieved. It basically relies on an "arrest-and-release" strategy using aphidicolin, an inhibitor of DNA replication, and propyzamide, a plant-microtubule disruptant. In a single-step process using aphidicolin alone, a cell population with about 70% of the cells at mitosis can be achieved, whereas by a two-step method using the two inhibitors sequentially, the level of synchrony can reach over 90%. The method of choice depends not only on the peak mitotic cell proportion but also on the cell cycle stage that is targeted for analysis. Both procedures take about 1.5 days, and cell cycle progression can be observed from the S phase to the next G1 phase at about 12 h after a 24 h-period treatment with aphidicolin.
Fumi Kumagai-Sano; Tomomi Hayashi; Toshio Sano; Seiichiro Hasezawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nature protocols     Volume:  1     ISSN:  1750-2799     ISO Abbreviation:  Nat Protoc     Publication Date:  2006  
Date Detail:
Created Date:  2007-04-04     Completed Date:  2007-11-05     Revised Date:  2008-03-24    
Medline Journal Info:
Nlm Unique ID:  101284307     Medline TA:  Nat Protoc     Country:  England    
Other Details:
Languages:  eng     Pagination:  2621-7     Citation Subset:  IM    
Department of Science Education, Faculty of Education, Gunma University, Aramaki-cho 4-2, Maebashi, Gunma 371-8510, Japan.
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MeSH Terms
Cell Culture Techniques*
Cell Cycle*
Cell Line
DNA, Plant / analysis
Tobacco / cytology*
Reg. No./Substance:
0/DNA, Plant

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