Document Detail


Cell cycle synchronization at the G2/M phase border by reversible inhibition of CDK1.
MedLine Citation:
PMID:  17172841     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chemical agents for cell cycle synchronization have greatly facilitated the study of biochemical events driving cell cycle progression. G1, S and M phase inhibitors have been developed and used widely in cell cycle research. However, currently there are no effective G2 phase inhibitors and synchronization of cultured cells in G2 phase has been challenging. Recently, a selective CDK1 inhibitor, RO-3306, has been identified that reversibly arrests proliferating human cells at the G2/M phase border and provides a novel means for cell cycle synchronization. A single-step protocol using RO-3306 permits the synchronization of >95% of cycling cancer cells in G2 phase. RO-3306 arrested cells enter mitosis rapidly after release from the G2 block thus allowing for isolation of mitotic cells without microtubule poisons. RO-3306 represents a new molecular tool for studying CDK1 function in human cells.
Authors:
Lyubomir T Vassilev
Publication Detail:
Type:  Journal Article     Date:  2006-11-15
Journal Detail:
Title:  Cell cycle (Georgetown, Tex.)     Volume:  5     ISSN:  1551-4005     ISO Abbreviation:  Cell Cycle     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-12-25     Completed Date:  2007-02-12     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  101137841     Medline TA:  Cell Cycle     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2555-6     Citation Subset:  IM    
Affiliation:
Discovery Oncology, Roche Research Center, Hoffmann-La Roche Inc., Nutley, New Jersey 07110, USA. lyubomir.vassilev@roche.com
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MeSH Terms
Descriptor/Qualifier:
Animals
CDC2 Protein Kinase / antagonists & inhibitors*,  metabolism
Cell Division
G2 Phase / drug effects*
Humans
Protein Kinase Inhibitors / pharmacology*
Quinolines / pharmacology*
Thiazoles / pharmacology*
Chemical
Reg. No./Substance:
0/Protein Kinase Inhibitors; 0/Quinolines; 0/RO 3306; 0/Thiazoles; EC 2.7.11.22/CDC2 Protein Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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