| Cell cycle synchronization of animal cells and nuclei by centrifugal elutriation. | |
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MedLine Citation:
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PMID: 18388949 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Synchronization of cells and nuclei is a powerful technique for the exact study of regulatory mechanisms and for understanding cell cycle events. Counterflow centrifugal elutriation is a biophysical cell separation technique in which cell size and sedimentation density differences of living cells are exploited to isolate subpopulations in various stages of cell cycle. Here, a protocol is described for the separation of phase-enriched subpopulations from exponentially growing Chinese hamster ovary cells at high-resolution power of elutriation. The efficiency of elutriation is confirmed by measuring the DNA content fluorimetrically and by flow cytometry. The resolution power of elutriation is demonstrated by the ability to fractionate nuclei of murine pre-B cells. The installation and elutriation by collecting 16-30 synchronized fractions, including particle size analysis, can be achieved in 4-5 h. |
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Authors:
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Gaspar Banfalvi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Nature protocols Volume: 3 ISSN: 1750-2799 ISO Abbreviation: Nat Protoc Publication Date: 2008 |
Date Detail:
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Created Date: 2008-04-04 Completed Date: 2008-10-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101284307 Medline TA: Nat Protoc Country: England |
Other Details:
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Languages: eng Pagination: 663-73 Citation Subset: IM |
Affiliation:
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Department of Microbial Biotechnology and Cell Biology, University of Debrecen, Debrecen 4010, Hungary. bgaspar@delfin.klte.hu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle / physiology* Cell Division Cell Line Cell Nucleus / physiology* Cell Separation / methods Centrifugation, Density Gradient / methods* Cricetinae Drosophila Epithelial Cells / cytology* Humans Mice |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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