| Cell cycle-specific replication of Escherichia coli minichromosomes. | |
| | |
MedLine Citation:
|
PMID: 3523483 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The timing of Escherichia coli minichromosome replication in the cell division cycle was examined using an improved procedure for studying plasmid replication frequency. Cultures growing exponentially in glucose/Casamino acids minimal medium were pulse-labeled with [3H]thymidine, and the radioactivity incorporated into plasmid DNA in cells of different ages was analyzed. At the end of the labeling period the bacteria were bound to the surface of a nitrocellulose membrane filter, and the radioactivity in new daughter cells, which eluted continuously from the membrane, was quantitated following agarose gel electrophoresis. The minichromosomes replicated during a discrete interval in the cell division cycle that appeared to coincide with initiation of chromosome replication. In contrast, plasmid pBR322 replicated throughout the division cycle at a rate that increased gradually as a function of cell age. The difference in minichromosome and pBR322 replication was clearly discernible in cells harboring both plasmids. It was also found that the 16 kD gene adjacent to oriC was not a determinant of the timing of minichromosome replication during the division cycle. The results are consistent with the conclusion that minichromosome replication frequency is governed by the same mechanism that controls chromosome replication. |
| | |
Authors:
|
A C Leonard; C E Helmstetter |
Related Documents
:
|
15939403 - Replicative aging of the yeast does not require dna replication. 21331633 - Production of ectoine through a combined process that uses both growing and resting cel... 2471073 - Specific mrnas in sertoli and germinal cells of testes from stage synchronized rats. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 83 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1986 Jul |
Date Detail:
|
Created Date: 1986-08-20 Completed Date: 1986-08-20 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 5101-5 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Cycle* Chromosomes, Bacterial / metabolism* DNA Replication* DNA, Bacterial / biosynthesis Escherichia coli / genetics, metabolism* Genes, Bacterial Plasmids* |
| Grant Support | |
ID/Acronym/Agency:
|
GM 26429/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/DNA, Bacterial |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Intracellular site of insulin action: mitochondrial Krebs cycle.
Next Document: Mutants of Salmonella typhimurium that cannot survive within the macrophage are avirulent.