| Cell-cycle-specific F plasmid replication: regulation by cell size control of initiation. | |
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MedLine Citation:
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PMID: 2013579 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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F plasmid replication during the Escherichia coli division cycle was investigated by using the membrane-elution technique to produce cells labeled at different times during the division cycle and scintillation counting for quantitative analysis of radioactive plasmid DNA. The F plasmid replicated, like the minichromosome, during a restricted portion of the bacterial division cycle; i.e., F plasmid replication is cell-cycle specific. The F plasmid replicated at a different time during the division cycle than a minichromosome present in the same cell. F plasmid replication coincided with doubling in the rate of enzyme synthesis from a plasmid-encoded gene. When the cell cycle age of replication of the F plasmid was determined over a range of growth rates, the cell size at which the F plasmid replicated followed the same rules as did replication of the bacterial chromosome--initiation occurred when a constant mass per origin was achieved--except that the initiation mass per origin for the F plasmid was different from that for the chromosome origin. In contrast, the high-copy mini-R6K plasmid replicated throughout the division cycle. |
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Authors:
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J D Keasling; B O Palsson; S Cooper |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of bacteriology Volume: 173 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1991 Apr |
Date Detail:
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Created Date: 1991-05-14 Completed Date: 1991-05-14 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2673-80 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109-0620. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle
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physiology* DNA Replication* Electrophoresis, Agar Gel F Factor / physiology* Humans |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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