Document Detail


Cell cycle sensing of oxidative stress in Saccharomyces cerevisiae by oxidation of a specific cysteine residue in the transcription factor Swi6p.
MedLine Citation:
PMID:  21147769     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Yeast cells begin to bud and enter the S phase when growth conditions are favorable during the G(1) phase. When subjected to some oxidative stresses, cells delay entry at G(1), allowing repair of cellular damage. Hence, oxidative stress sensing is coordinated with the regulation of cell cycle. We identified a novel function of the cell cycle regulator of Saccharomyces cerevisiae, Swi6p, as a redox sensor through its cysteine residue at position 404. When alanine was substituted at this position, the resultant mutant, C404A, was sensitive to several reactive oxygen species and oxidants including linoleic acid hydroperoxide, the superoxide anion, and diamide. This mutant lost the ability to arrest in G(1) phase upon treatment with lipid hydroperoxide. The Cys-404 residue of Swi6p in wild-type cells was oxidized to a sulfenic acid when cells were subjected to linoleic acid hydroperoxide. Mutation of Cys-404 to Ala abolished the down-regulation of expression of the G(1) cyclin genes CLN1, CLN2, PCL1, and PCL2 that occurred when cells of the wild type were exposed to the lipid hydroperoxide. In conclusion, oxidative stress signaling for cell cycle regulation occurs through oxidation of the G(1)/S-specific transcription factor Swi6p and consequently leads to suppression of the expression of G(1) cyclins and a delay in cells entering the cell cycle.
Authors:
Joyce Chiu; Carole M Tactacan; Shi-Xiong Tan; Ruby C Y Lin; Merridee A Wouters; Ian W Dawes
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural     Date:  2010-12-08
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  286     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-02-14     Completed Date:  2011-04-11     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5204-14     Citation Subset:  IM    
Affiliation:
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales 2052, Australia.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Substitution
Cyclins
Cysteine / genetics,  metabolism
G1 Phase / physiology*
Gene Expression Regulation, Fungal / physiology
Lipid Peroxides / metabolism
Mutation, Missense
Oxidation-Reduction
Oxidative Stress / physiology*
S Phase / physiology*
Saccharomyces cerevisiae / genetics,  metabolism*
Saccharomyces cerevisiae Proteins / genetics,  metabolism*
Signal Transduction / physiology*
Transcription Factors / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Cyclins; 0/Lipid Peroxides; 0/SWI6 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 0/Transcription Factors; 52-90-4/Cysteine
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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