| Cell-cycle research with synchronous cultures: an evaluation. | |
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MedLine Citation:
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PMID: 11254979 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The baby-machine system, which produces new-born Escherichia coli cells from cultures immobilized on a membrane, was developed many years ago in an attempt to attain optimal synchrony with minimal disturbance of steady-state growth. In the present article, we put forward a model to describe the behaviour of cells produced by this method, and provide quantitative evaluation of the parameters involved, at each of four different growth rates. Considering the high level of selection achievable with this technique and the natural dispersion in interdivision times, we believe that the output of the baby machine is probably close to optimal in terms of both quality and persistence of synchrony. We show that considerable information on events in the cell cycle can be obtained from populations with age distributions very much broader than those achieved with the baby machine and differing only modestly from steady state. The data presented here, together with the long and fruitful history of findings employing the baby-machine technique, suggest that minimisation of stress on cells is the single most important factor for successful cell-cycle analysis. |
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Authors:
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C E Helmstetter; M Thornton; N B Grover |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Biochimie Volume: 83 ISSN: 0300-9084 ISO Abbreviation: Biochimie Publication Date: 2001 Jan |
Date Detail:
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Created Date: 2001-03-20 Completed Date: 2001-06-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1264604 Medline TA: Biochimie Country: France |
Other Details:
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Languages: eng Pagination: 83-9 Citation Subset: IM; S |
Affiliation:
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Department of Biological Sciences, Florida Institute of Technology, Melbourne 32901, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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metabolism Cell Cycle* Cell Division* Cells, Immobilized Culture Media / chemistry Escherichia coli / cytology, genetics, growth & development, physiology* Glucose / metabolism Models, Biological |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Culture Media; 50-99-7/Glucose |
| Investigator | |
Investigator/Affiliation:
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C E Helmstetter / FL Inst Tech, Melbourne |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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