Document Detail

Cell cycle requirements in assembling silent chromatin in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  16428441     Owner:  NLM     Status:  MEDLINE    
The establishment of silencing at the silent mating-type locus, HMR, in Saccharomyces cerevisiae requires that yeast pass through S phase of the cell cycle, yet requires neither the initiation of DNA replication at the locus destined to become silenced nor the passage of a replication fork through that locus. We tested whether this S-phase requirement reflects a window within the cell cycle permissive for recruitment of Sir proteins to HMR. The S-phase-restricted event necessary for silencing occurred after recruitment of Sir proteins to HMR. Moreover, cells arrested in early S phase formed silent chromatin at HMR, provided HMR was on a nonreplicating template. Replicating templates required a later step for silencing. These results provide temporal resolution of discrete steps in the formation of silent chromatin and suggest that more than one cell cycle-regulated event may be necessary for the establishment of silencing.
Ann L Kirchmaier; Jasper Rine
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  26     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-01-23     Completed Date:  2006-02-28     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  852-62     Citation Subset:  IM    
Department of Biochemistry and Purdue Cancer Center, Purdue University, 175 S. University St., West Lafayette, IN 47907, USA.
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MeSH Terms
Cell Cycle*
Chromatin / metabolism*
DNA Replication
G1 Phase
Gene Expression Regulation, Fungal*
Gene Silencing*
Genes, Mating Type, Fungal
Histone Deacetylases / genetics,  metabolism
RNA Stability
S Phase
Saccharomyces cerevisiae / genetics*,  metabolism
Silent Information Regulator Proteins, Saccharomyces cerevisiae / genetics,  metabolism*
Sirtuin 2
Sirtuins / genetics,  metabolism
Grant Support
Reg. No./Substance:
0/Chromatin; 0/Silent Information Regulator Proteins, Saccharomyces cerevisiae; EC 3.5.1.-/SIR2 protein, S cerevisiae; EC 3.5.1.-/Sirtuin 2; EC 3.5.1.-/Sirtuins; EC Deacetylases

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