Document Detail

Cell cycle-related transformation of the E2F4-p130 repressor complex.
MedLine Citation:
PMID:  16153605     Owner:  NLM     Status:  MEDLINE    
During G0 phase the p130, member of the pRb tumor suppressor protein family, forms a repressor complex with E2F4 which is inactivated in G1/S by hyperphosphorylation of the p130. The role of p130 after G1/S remains poorly investigated. We found that in nuclear extracts of T98G cells, the p130-E2F4-DNA (pp-E2F4) complex does not dissociate at G1/S transition, but instead reverts to the p130-E2F4-cyclin E/A-cdk2 (cyc/cdk-pp-E2F4) complex, which is detected in S and G2/M phases of the cell cycle. Hyperphosphorylation of the p130 at G1/S transition is associated with a decrease of its total amount; however, this protein is still detected during the rest of the cell cycle, and it is increasingly hyperphosphorylated in the cytosol, but continuously dephosphorylated in the nucleus. Both nuclear and cytosol cell fractions in T98G cells contain a hyperphosphorylated form of p130 in complex with E2F4 at S and G2/M cell cycle phases. In contrast to T98G cells, transformation of the p130 containing cyc/cdk-pp-E2F4 complex into the p130-pp-E2F4 repressor does not occur in HeLa cells under growth restriction conditions.
Boris Popov; Long-Sheng Chang; Vladimir Serikov
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  336     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-09-23     Completed Date:  2005-11-01     Revised Date:  2012-06-25    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  762-9     Citation Subset:  IM    
Institute of Cytology, Russian Academy of Sciences, 4, Tikhoretsky Ave., St. Petersburg 194064, Russia.
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MeSH Terms
CDC2-CDC28 Kinases / metabolism
Cell Cycle*
Cell Line, Tumor
Cell Nucleus / metabolism
Cyclin-Dependent Kinase 2
Cytoplasm / metabolism
DNA-Binding Proteins / metabolism*
E2F4 Transcription Factor
HeLa Cells
Proteins / metabolism*
Repressor Proteins / metabolism*
Retinoblastoma-Like Protein p130
Transcription Factors / metabolism*
Grant Support
Reg. No./Substance:
0/DNA-Binding Proteins; 0/E2F4 Transcription Factor; 0/Proteins; 0/Repressor Proteins; 0/Retinoblastoma-Like Protein p130; 0/Transcription Factors; EC Kinases; EC protein, human; EC Kinase 2

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