| Cell cycle-related changes in F-actin distribution are correlated with glycolytic activity. | |
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MedLine Citation:
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PMID: 7796889 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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XTH-2 cells, a cell line derived from tadpole heart endothelial cells, were blocked at the end of the G1 phase of the cell cycle using desoxyguanosine (dG). Stress fibers are the dominant actin fibril structure in blocked cells. They disappear starting with the release of the dG block until the G2 phase of the cell cycle. In addition, after the release peripheral lamellae and microspikes appear, indicating increased surface motility of the cells. Concomitantly with these changes, net lactic acid production is increased while oxygen consumption remains constant. Increase in the content of F-actin per cell takes place only in subconfluent cultures. Similar morphological changes (organization of F-actin) have been induced by phorbol myristate acetate treatment. These are also accompanied by an increase in lactic acid production and unaltered oxygen consumption. Therefore, the changes in energy metabolism are supposed to result from the rearrangement of the F-actin network: The emanating loose fibrillar pattern provides an increased surface for the association with glycolytic enzymes, which enhances enzyme activity and represents a more motile state. The increased energy demand of the more motile structures is supplied predominantly by ATP derived from glycolysis. |
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Authors:
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J Bereiter-Hahn; C Stübig; V Heymann |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental cell research Volume: 218 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 1995 Jun |
Date Detail:
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Created Date: 1995-07-28 Completed Date: 1995-07-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 551-60 Citation Subset: IM |
Affiliation:
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Biozentrum, Johann Wolfgang Goethe-Universität, Frankfurt am Main, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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analysis* Animals Anura / embryology, physiology* Cell Cycle / physiology Embryo, Nonmammalian / physiology Energy Metabolism / drug effects Glycolysis Lactates / biosynthesis* Lactic Acid Phorbol Esters / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Lactates; 0/Phorbol Esters; 50-21-5/Lactic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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