Document Detail


Cell cycle regulation of histone H1O in CHO cells: a flow cytofluorimetric study after double staining of the cells.
MedLine Citation:
PMID:  6641718     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The regulation of histone H1O content throughout the cell cycle of non-synchronized Chinese hamster ovary (CHO) cells has been studied using double fluorescent staining and flow cytofluorometry. In exponentially growing cells, the amount of H1O was found to be proportional to the DNA content of the cells, indicating that the protein is synthesized throughout the cell cycle. However, when cells were arrested in G1 at saturation density the amount of H1O was greater than that found in G1 cells of the exponentially growing population. In contrast, the levels of H1-1 were the same for G1 cells of both populations. These results show that the regulation of H1O accumulation differs from that of other histones.
Authors:
A Chabanas; J J Lawrence; J Humbert; H Eisen
Related Documents :
6467328 - Progressive increase in polyamine levels in 9l cells in vitro during the cell cycle: co...
1673788 - Cell cycle-specific effects of lovastatin.
19024658 - Enhanced use of backup pathways of nhej in g2 in chinese hamster mutant cells with defe...
448638 - Arrest of cultured cells in the g1 phase of the cell cycle by indomethacin.
25458008 - A longer life for yeast with good memory.
21846468 - Ago1 and ago2 differentially affect cell proliferation, motility and apoptosis when ove...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The EMBO journal     Volume:  2     ISSN:  0261-4189     ISO Abbreviation:  EMBO J.     Publication Date:  1983  
Date Detail:
Created Date:  1984-01-07     Completed Date:  1984-01-07     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  833-7     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle*
Cell Division
Cells, Cultured
Cricetinae
Cricetulus
DNA / metabolism
Female
Flow Cytometry
Fluorescent Dyes
Histones / metabolism*
Ovary
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Histones; 9007-49-2/DNA
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Polynucleotide-histone H1 complexes as probes for blot hybridization.
Next Document:  Shuffling adenovirus promoters: a viral recombinant with early region 1A under late transcriptional ...