Document Detail

Cell cycle regulation of histone H1O in CHO cells: a flow cytofluorimetric study after double staining of the cells.
MedLine Citation:
PMID:  6641718     Owner:  NLM     Status:  MEDLINE    
The regulation of histone H1O content throughout the cell cycle of non-synchronized Chinese hamster ovary (CHO) cells has been studied using double fluorescent staining and flow cytofluorometry. In exponentially growing cells, the amount of H1O was found to be proportional to the DNA content of the cells, indicating that the protein is synthesized throughout the cell cycle. However, when cells were arrested in G1 at saturation density the amount of H1O was greater than that found in G1 cells of the exponentially growing population. In contrast, the levels of H1-1 were the same for G1 cells of both populations. These results show that the regulation of H1O accumulation differs from that of other histones.
A Chabanas; J J Lawrence; J Humbert; H Eisen
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The EMBO journal     Volume:  2     ISSN:  0261-4189     ISO Abbreviation:  EMBO J.     Publication Date:  1983  
Date Detail:
Created Date:  1984-01-07     Completed Date:  1984-01-07     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  833-7     Citation Subset:  IM    
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MeSH Terms
Cell Cycle*
Cell Division
Cells, Cultured
DNA / metabolism
Flow Cytometry
Fluorescent Dyes
Histones / metabolism*
Reg. No./Substance:
0/Fluorescent Dyes; 0/Histones; 9007-49-2/DNA

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