Document Detail

Cell cycle regulation of hepatitis C and encephalomyocarditis virus internal ribosome entry site-mediated translation in human embryonic kidney 293 cells.
MedLine Citation:
PMID:  12902037     Owner:  NLM     Status:  MEDLINE    
We have established stably transformed human embryonic kidney cell lines (HEK293) containing bicistronic constructs to study regulation of viral internal ribosome entry site (IRES)-mediated translation in vivo. These cells produce Renilla luciferase (Rluc) in a cap-dependent manner, while Firefly luciferase (Luc) synthesis is mediated by IRES elements. Using these cell lines, we demonstrate here that IRES-mediated translation directed by both hepatitis C (HCV) and encephalomyocarditis (EMCV) virus varies with the cell cycle. Experiments involving arrest of the cell lines at different phases of the cell cycle, release of synchronized cells from cell cycle arrest, as well as direct sorting of the cells based on position in the cell cycle have shown that the activity of the HCV and EMCV IRES elements is lowest during the G2/M phase in HEK293 cells. These results suggest that cellular trans-acting factors either stimulate viral IRES-mediated translation during G1 and S phases or repress translation during the G2/M phase in HEK293 cells.
Arun Venkatesan; Rakhi Sharma; Asim Dasgupta
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Virus research     Volume:  94     ISSN:  0168-1702     ISO Abbreviation:  Virus Res.     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-08-06     Completed Date:  2003-10-02     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8410979     Medline TA:  Virus Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  85-95     Citation Subset:  IM    
Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA.
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MeSH Terms
5' Untranslated Regions*
Cell Cycle / physiology*
Cell Line, Transformed
DNA, Recombinant
Encephalomyocarditis virus / genetics*,  physiology
Gene Expression Regulation, Viral
Genes, Reporter
Hepacivirus / genetics*,  physiology
Luciferases / biosynthesis,  metabolism
Protein Biosynthesis*
Regulatory Sequences, Ribonucleic Acid*
Transformation, Genetic
Grant Support
Reg. No./Substance:
0/5' Untranslated Regions; 0/DNA, Recombinant; 0/Regulatory Sequences, Ribonucleic Acid; EC 1.13.12.-/Luciferases

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