| Cell cycle regulation of hepatitis C virus internal ribosomal entry site-directed translation. | |
| | |
MedLine Citation:
|
PMID: 10611164 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND & AIMS: Translation of the hepatitis C virus (HCV) polyprotein is mediated by an internal ribosome entry site (IRES) that is located within the 5' nontranslated segment of the viral RNA. This RNA segment is known to form binary complexes with isolated 40S ribosome subunits in vitro, but there is little understanding of how the process of virus translation is regulated in vivo. METHODS: We established 2 stably transformed cell lines from Huh-7 cells that constitutively express dicistronic RNA transcripts containing sequences encoding 2 reporter proteins (Renilla luciferase and firefly luciferase) separated by a functional HCV IRES. The translation of the upstream Renilla luciferase reading frame is initiated in these cells by the usual cellular cap-dependent mechanism, whereas translation of the downstream firefly luciferase reading frame is initiated by the IRES. RESULTS: Compared with cap-dependent translation, the activity of the IRES was greatest in actively growing cells and relatively reduced in resting cells. In synchronized cultures of these stably transformed cells, the IRES activity varied with the cell cycle and was greatest during the mitotic (M) phases and lowest during the quiescent (G(0)) phases. CONCLUSIONS: These findings suggest that HCV translation is regulated by cellular proteins that vary in abundance during the cell cycle and that viral translation may be enhanced by factors that stimulate the regeneration of hepatocytes in patients with chronic hepatitis C. |
| | |
Authors:
|
M Honda; S Kaneko; E Matsushita; K Kobayashi; G A Abell; S M Lemon |
Related Documents
:
|
22401854 - Mitochondrial superoxide production contribute to vancomycin-induced renal tubular cell... 6174324 - Selective inhibition by sodium butyrate of glucocorticoid-induced tyrosine aminotransfe... 20581224 - An alu-like rna promotes cell differentiation and reduces malignancy of human neuroblas... 1277134 - Comparison of the products of the reaction of 7-methylbenz(a)anthracene 5,6-oxide and r... 18298454 - Cell wall fraction of enterococcus hirae ameliorates tnf-alpha-induced barrier impairme... 2416424 - Expression of mallory bodies in hepatocellular carcinoma in man and its significance. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Gastroenterology Volume: 118 ISSN: 0016-5085 ISO Abbreviation: Gastroenterology Publication Date: 2000 Jan |
Date Detail:
|
Created Date: 2000-01-19 Completed Date: 2000-01-19 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0374630 Medline TA: Gastroenterology Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 152-62 Citation Subset: AIM; IM |
Affiliation:
|
First Department of Internal Medicine, Kanazawa University, Kanazawa, Japan. mhonda@medf.m.kanazawa-u.ac.jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
5' Untranslated Regions
/
physiology* Animals Beetles / enzymology Cell Cycle / physiology* Cell Line, Transformed Cycloheximide / pharmacology Genes, Reporter Hepacivirus / genetics* Luciferases / metabolism Protein Biosynthesis* RNA, Viral / genetics* Transcription, Genetic |
| Grant Support | |
ID/Acronym/Agency:
|
U19-AI40035/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/5' Untranslated Regions; 0/RNA, Viral; 66-81-9/Cycloheximide; EC 1.13.12.-/Luciferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Vitamin D-receptor genotypes as independent genetic predictors of decreased bone mineral density in ...
Next Document: Expression of the bile salt export pump is maintained after chronic cholestasis in the rat.