Document Detail


Cell cycle regulation during growth-dormancy cycles in pea axillary buds.
MedLine Citation:
PMID:  7579177     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Accumulation patterns of mRNAs corresponding to histones H2A and H4, ribosomal protein genes rpL27 and rpL34, MAP kinase, cdc2 kinase and cyclin B were analyzed during growth-dormancy cycles in pea (Pisum sativum cv. Alaska) axillary buds. The level of each of these mRNAs was low in dormant buds on intact plants, increased when buds were stimulated to grow by decapitating the terminal bud, decreased when buds ceased growing and became dormant, and then increased when buds began to grow again. Flow cytometry was used to determine nuclear DNA content during these developmental transitions. Dormant buds contain G1 and G2 nuclei (about 3:1 ratio), but only low levels of S phase nuclei. It is hypothesized that cells in dormant buds are arrested at three points in the cell cycle, in mid-G1, at the G1/S boundary and near the S/G2 boundary. Based on the accumulation of histone H2A and H4 mRNAs, which are markers for S phase, cells arrested at the G1/S boundary enter S within one hour of decapitation. The presence of a cell population arrested in mid-G1 is indicated by a second peak of histone mRNA accumulation 6 h after the first peak. Based on the accumulation of cyclin B mRNA, a marker for late G2 and mitosis, cells arrested at G1/S begin to divide between 12 and 18 h after decapitation. A small increase in the level of cyclin B mRNA at 6 h after decapitation may represent mitosis of the cells that has been arrested near the S/G2 boundary. Accumulation of MAP kinase, cdc2 kinase, rpL27 and rpL34 mRNAs are correlated with cell proliferation but not with a particular phase of the cell cycle.
Authors:
M L Devitt; J P Stafstrom
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plant molecular biology     Volume:  29     ISSN:  0167-4412     ISO Abbreviation:  Plant Mol. Biol.     Publication Date:  1995 Oct 
Date Detail:
Created Date:  1995-12-04     Completed Date:  1995-12-04     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9106343     Medline TA:  Plant Mol Biol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  255-65     Citation Subset:  IM    
Affiliation:
Plant Molecular Biology Center, Northern Illinois University, DeKalb 60115, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/U10041;  U10042;  U10043;  U10044;  U10045;  U10046;  U10047
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Blotting, Northern
CDC2 Protein Kinase / genetics
Calcium-Calmodulin-Dependent Protein Kinases / genetics
Cell Cycle*
Cell Separation
Cloning, Molecular
DNA, Complementary / genetics
DNA, Plant / analysis
Flow Cytometry
Histones / genetics
Molecular Sequence Data
Peas / cytology,  genetics,  growth & development*
Plant Stems / cytology,  genetics,  growth & development*
RNA, Messenger / analysis
Ribosomal Proteins / genetics
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/DNA, Plant; 0/Histones; 0/RNA, Messenger; 0/Ribosomal Proteins; 0/ribosomal protein L34; 0/ribosomal proteins L27; EC 2.7.11.17/Calcium-Calmodulin-Dependent Protein Kinases; EC 2.7.11.22/CDC2 Protein Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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