Document Detail


Cell cycle regulation of the c-Myc transcriptional activation domain.
MedLine Citation:
PMID:  8321217     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The product of the c-myc gene (c-Myc) is a sequence-specific DNA-binding protein that has previously been demonstrated to be required for cell cycle progression. Here we report that the c-Myc DNA binding site confers cell cycle regulation to a reporter gene in Chinese hamster ovary cells. The observed transactivation was biphasic with a small increase in G1 and a marked increase during the S-to-G2/M transition of the cell cycle. This cell cycle regulation of transactivation potential is accounted for, in part, by regulatory phosphorylation of the c-Myc transactivation domain. Together, these data demonstrate that c-Myc may have an important role in the progression of cells through both the G1 and G2 phases of the cell cycle.
Authors:
A Seth; S Gupta; R J Davis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  13     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  1993 Jul 
Date Detail:
Created Date:  1993-07-30     Completed Date:  1993-07-30     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4125-36     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester 01605.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Binding Sites
CHO Cells
Cell Cycle / genetics*
Cell Line
Cricetinae
DNA
Gene Expression Regulation
Genes, myc*
Kinetics
Molecular Sequence Data
Phosphorylation
Serine / metabolism
Transcription, Genetic*
Transcriptional Activation
Grant Support
ID/Acronym/Agency:
CA58396/CA/NCI NIH HHS; GM37845/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
56-45-1/Serine; 9007-49-2/DNA
Comments/Corrections

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