Document Detail


Cell cycle regulation by light in Prochlorococcus strains.
MedLine Citation:
PMID:  11157244     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of light on the synchronization of cell cycling was investigated in several strains of the oceanic photosynthetic prokaryote Prochlorococcus using flow cytometry. When exposed to a light-dark (L-D) cycle with an irradiance of 25 micromol of quanta x m(-2) x s(-1), the low-light-adapted strain SS 120 appeared to be better synchronized than the high-light-adapted strain PCC 9511. Submitting L-D-entrained populations to shifts (advances or delays) in the timing of the "light on" signal translated to corresponding shifts in the initiation of the S phase, suggesting that this signal is a key parameter for the synchronization of population cell cycles. Cultures that were shifted from an L-D cycle to continuous irradiance showed persistent diel oscillations of flow-cytometric signals (light scatter and chlorophyll fluorescence) but with significantly reduced amplitudes and a phase shift. Complete darkness arrested most of the cells in the G1 phase of the cell cycle, indicating that light is required to trigger the initiation of DNA replication and cell division. However, some cells also arrested in the S phase, suggesting that cell cycle controls in Prochlorococcus spp. are not as strict as in marine Synechococcus spp. Shifting Prochlorococcus cells from low to high irradiance translated quasi-instantaneously into an increase of cells in both the S and G2 phases of the cell cycle and then into faster growth, whereas the inverse shift induced rapid slowing of the population growth rate. These data suggest a close coupling between irradiance levels and cell cycling in Prochlorococcus spp.
Authors:
S Jacquet; F Partensky; D Marie; R Casotti; D Vaulot
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  67     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-07-05     Revised Date:  2013-06-11    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  782-90     Citation Subset:  IM    
Affiliation:
Station Biologique, CNRS, INSU and Université Pierre-et-Marie-Curie, BP 74 F-29682 Roscoff, France. nimsj@im.uib.no
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle / physiology*
Circadian Rhythm
Culture Media
Cyanobacteria / cytology,  growth & development*
Darkness
Flow Cytometry
Gene Expression Regulation, Bacterial*
Light*
Chemical
Reg. No./Substance:
0/Culture Media
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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