Document Detail


Cell cycle-regulated centers of DNA double-strand break repair.
MedLine Citation:
PMID:  12963848     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In eukaryotes, homologous recombination is an important pathway for the repair of DNA double-strand breaks. We have studied this process in living cells in the yeast Saccharomyces cerevisiae using Rad52 as a cell biological marker. In response to DNA damage, Rad52 redistributes itself and forms foci specifically during S phase. We have shown previously that Rad52 foci are centers of DNA repair where multiple DNA double-strand breaks colocalize. Here we report a correlation between the timing of Rad52 focus formation and modification of the Rad52 protein. In addition, we show that the two ends of a double-strand break are held tightly together in the majority of cells. Interestingly, in a small but significant fraction of the S phase cells, the two ends of a break separate suggesting that mechanisms exist to reassociate and align these ends for proper DNA repair.
Authors:
Michael Lisby; Adriana Antúnez de Mayolo; Uffe H Mortensen; Rodney Rothstein
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell cycle (Georgetown, Tex.)     Volume:  2     ISSN:  1538-4101     ISO Abbreviation:  Cell Cycle     Publication Date:    2003 Sep-Oct
Date Detail:
Created Date:  2003-09-09     Completed Date:  2004-05-11     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  101137841     Medline TA:  Cell Cycle     Country:  United States    
Other Details:
Languages:  eng     Pagination:  479-83     Citation Subset:  IM    
Affiliation:
Department of Genetics and Development, Columbia University, College of Physicians and Surgeons, New York, New York 10032-2704, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle / genetics,  physiology*
DNA Damage / genetics,  physiology*
DNA Repair / genetics,  physiology*
DNA-Binding Proteins / genetics,  metabolism
Rad52 DNA Repair and Recombination Protein
Recombination, Genetic / physiology*
S Phase / physiology
Saccharomyces cerevisiae / genetics,  physiology
Saccharomyces cerevisiae Proteins
Grant Support
ID/Acronym/Agency:
GM50237/GM/NIGMS NIH HHS; GM67055/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/RAD52 protein, S cerevisiae; 0/Rad52 DNA Repair and Recombination Protein; 0/Saccharomyces cerevisiae Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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