| Cell cycle progression is required for zebrafish somite morphogenesis but not segmentation clock function. | |
| | |
MedLine Citation:
|
PMID: 18480162 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cell division, differentiation and morphogenesis are coordinated during embryonic development, and frequently are in disarray in pathologies such as cancer. Here, we present a zebrafish mutant that ceases mitosis at the beginning of gastrulation, but that undergoes axis elongation and develops blood, muscle and a beating heart. We identify the mutation as being in early mitotic inhibitor 1 (emi1), a negative regulator of the Anaphase Promoting Complex, and use the mutant to examine the role of the cell cycle in somitogenesis. The mutant phenotype indicates that axis elongation during the segmentation period is driven substantially by cell migration. We find that the segmentation clock, which regulates somitogenesis, functions normally in the absence of cell cycle progression, and observe that mitosis is a modest source of noise for the clock. Somite morphogenesis involves the epithelialization of the somite border cells around a core of mesenchyme. As in wild-type embryos, somite boundary cells are polarized along a Fibronectin matrix in emi1(-/-). The mutants also display evidence of segment polarity. However, in the absence of a normal cell cycle, somites appear to hyper-epithelialize, as the internal mesenchymal cells exit the core of the somite after initial boundary formation. Thus, cell cycle progression is not required during the segmentation period for segmentation clock function but is necessary for the normal segmental arrangement of epithelial borders and internal mesenchymal cells. |
| | |
Authors:
|
Lixia Zhang; Christina Kendrick; Dörthe Jülich; Scott A Holley |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2008-05-14 |
Journal Detail:
|
Title: Development (Cambridge, England) Volume: 135 ISSN: 0950-1991 ISO Abbreviation: Development Publication Date: 2008 Jun |
Date Detail:
|
Created Date: 2008-05-28 Completed Date: 2008-08-19 Revised Date: 2010-09-21 |
Medline Journal Info:
|
Nlm Unique ID: 8701744 Medline TA: Development Country: England |
Other Details:
|
Languages: eng Pagination: 2065-70 Citation Subset: IM |
Affiliation:
|
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Body Patterning / physiology Cell Cycle / physiology* Cell Cycle Proteins / physiology* Embryo, Nonmammalian / physiology Morphogenesis* Mutation Somites / cytology, physiology* Zebrafish / embryology, genetics, physiology* Zebrafish Proteins / genetics*, metabolism, physiology |
| Grant Support | |
ID/Acronym/Agency:
|
R01 HD045738/HD/NICHD NIH HHS; R01 HD045738-04/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Cell Cycle Proteins; 0/Zebrafish Proteins; 0/emi1 protein, Zebrafish |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Tracheal branching morphogenesis in Drosophila: new insights into cell behaviour and organ architect...
Next Document: A maternally localised Wnt ligand required for axial patterning in the cnidarian Clytia hemisphaeric...