| Cell cycle progression is required for nuclear migration of neural progenitor cells. | |
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MedLine Citation:
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PMID: 16650835 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the developing brain, neural progenitor cells in the ventricular zone (VZ) show a typical migration pattern-interkinetic nuclear migration, in which nuclear position within the VZ is correlated with the cell cycle. However, the mechanisms underlying this regulation remain unclear. To clarify whether the cell cycle progression controls nuclear migration of neural progenitor cells, we determined whether chemically induced cell cycle arrest affected nuclear migration patterns in the VZ. Administration of 5-azacytidine (5AzC) or cyclophosphamide (CP) to pregnant mice induced cell cycle arrest in the fetal neural progenitor cells of the telencephalon: 5AzC induced G2/M-phase arrest, and CP induced S-phase arrest. We used 5-bromo-2'-deoxyuridine (BrdU) labeling to determine the position of the cell in the cell cycle and the nuclei within the VZ at the same time. Cells arrested in G2/M-phase stopped migrating in the inner area of the VZ. Cells arrested in S-phase stopped migrating in the outer area. These results indicate that nuclear position within the VZ was correlated with cell cycle phase, even when the cell cycle was disrupted, and that the nuclei of neural progenitor cells can migrate only when their cell cycle is going. Our results suggest that cell cycle regulators might control the machinery of migration through a common regulatory mechanism. |
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Authors:
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Masaki Ueno; Kei-ichi Katayama; Hirofumi Yamauchi; Hiroyuki Nakayama; Kunio Doi |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2006-05-02 |
Journal Detail:
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Title: Brain research Volume: 1088 ISSN: 0006-8993 ISO Abbreviation: Brain Res. Publication Date: 2006 May |
Date Detail:
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Created Date: 2006-05-24 Completed Date: 2006-08-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0045503 Medline TA: Brain Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 57-67 Citation Subset: IM |
Affiliation:
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Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. ms-ueno@umin.ac.jp |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects Azacitidine / pharmacology Bromodeoxyuridine / metabolism Cell Cycle / drug effects, physiology* Cell Movement / physiology* Cell Nucleus / physiology* Cyclophosphamide / pharmacology Enzyme Inhibitors / pharmacology Female Flow Cytometry / methods Immunohistochemistry / methods Immunosuppressive Agents / pharmacology In Situ Nick-End Labeling / methods Mice Mice, Inbred C57BL Neurons / cytology*, physiology Pregnancy Stem Cells / physiology* Telencephalon / cytology, embryology Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Immunosuppressive Agents; 320-67-2/Azacitidine; 50-18-0/Cyclophosphamide; 59-14-3/Bromodeoxyuridine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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